Description
PrymaLab · Research use only
GLOW BLEND 70MG
GHK-Cu 50 mg, BPC-157 10 mg and full-length TB-500 10 mg freeze-dried in one vial
GLOW is a three-peptide research blend of GHK-Cu, BPC-157 and TB-500, here in its 70 mg vial: 50 mg of the copper complex with 10 mg each of the other two, freeze-dried together into one blue cake. The TB-500 is full-length thymosin beta-4. Nobody has published research on GLOW as a blend; what follows is the literature on each peptide, from fibroblast collagen work to endothelial signalling and actin binding.
Specifications
| Property | Value |
|---|---|
| Components | GHK-Cu (Copper tripeptide-1; Gly-His-Lys · Cu(II)) BPC-157 (Body Protection Compound-157; pentadecapeptide) TB-500 (Thymosin β4); N-acetyl thymosin β4, full-length 43-residue peptide |
| GHK-Cu CAS number | 89030-95-5 |
| BPC-157 CAS number | 137525-51-0 |
| TB-500 (Thymosin β4) CAS number | 77591-33-4 |
| Label claim | GHK-Cu 50 mg + BPC-157 10 mg + TB-500 (Thymosin β4) 10 mg (70 mg in total) |
| Form | Lyophilized (freeze-dried) powder or cake in a sealed glass vial with a rubber stopper and aluminium crimp cap |
| Storage, sealed | −20 °C, desiccated, dark |
| Purity specification | 99.0% or higher by RP-HPLC |
| Lot certificate | Results for the lot now shipping are in the certificate panel on this page |
| Safety data sheet | PL-SDS-069 |
What is in the vial
The lot certificate confirms three components by LC-MS: GHK-Cu (the Gly-His-Lys copper(II) complex, 401.9 Da), BPC-157 (H-GEPPPGKPADDAGLV-OH, 1419.6 Da) and N-acetyl thymosin beta-4 (43 residues, 4963.5 Da). The mass ratio is 5:1:1. In molar terms the vial holds about 124 µmol of GHK-Cu, 7.0 µmol of BPC-157 and 2.0 µmol of thymosin beta-4, so the copper complex outnumbers the largest peptide by roughly sixty to one.
GLOW 50 mg is not a smaller fill of this vial. It holds 35 mg of GHK-Cu, 5 mg of BPC-157 and 10 mg of TB-500, a 7:1:2 ratio, so the two vials give different proportions at any dilution. The 5:1:1 ratio of this vial is the one used in the GLOW preloaded pen, and adding 10 mg of KPV to it gives KLOW.
What the research covers
GLOW has not been studied as a blend. No paper reports these three peptides given together to cells or animals, and none characterises the mixture, so the evidence below belongs to each component on its own.
GHK-Cu and fibroblasts
GHK, a tripeptide isolated from human plasma, binds copper(II) avidly1. Maquart and colleagues reported in 1988 that the copper complex stimulated collagen synthesis by cultured fibroblasts, beginning between 1 and 10 picomolar and reaching its maximum at 1 nanomolar, with no change in cell number1. Because the GHK sequence appears in type I collagen, they proposed that proteases at a wound could free it from the matrix1. Pickart and Margolina’s 2018 review lists further actions: increased elastin and glycosaminoglycan synthesis, blood vessel and nerve outgrowth, and repair of skin, lung connective tissue, bone, liver and stomach lining2.
BPC-157 in endothelial and tendon cells
BPC-157 is a 15-residue sequence derived from a protein in human gastric juice3. Hsieh and colleagues examined its effect on blood vessels in three systems. It increased vessel density in the chick chorioallantoic membrane and in endothelial tube formation assays, and it sped the return of blood flow to ischemic hind-limb muscle in rats4. In human vascular endothelial cells it raised VEGFR2 mRNA and protein but not VEGF-A, promoted internalisation of the receptor, and activated the VEGFR2-Akt-eNOS pathway over time; an inhibitor of endocytosis blocked these effects4. Chang and colleagues studied rat Achilles tendon. BPC-157 accelerated outgrowth from tendon explants and increased the migration and spreading of tendon fibroblasts, along with F-actin formation and phosphorylation of FAK and paxillin3. It improved survival under hydrogen peroxide stress but did not directly change proliferation3.
Thymosin beta-4 and actin
Thymosin beta-4 belongs to the beta-thymosins, highly conserved peptides of about 5 kDa first thought to be thymic hormones5. They turned out to be the main G-actin sequestering peptides in cells, binding monomeric actin one to one and acting as a buffer that releases monomers when filaments are needed5. Outside cells thymosin beta-4 occurs in plasma and wound fluid, and effects attributed to it include induction of metalloproteinases, chemotaxis and angiogenesis5.
What is not established
The component evidence is cell and animal work; the abstracts cited here report no human outcomes for any of the three. Huff and colleagues stated that nothing was known about the molecular mechanisms of thymosin beta-4 outside cells, and raised the possibility that higher beta-thymosin expression could aid metastasis by making cells more mobile5. Whether the three peptides interact, in solution or in a model, has not been tested. None of these papers used PrymaLab material. The GLOW peptide guide compares the depth of evidence for each component.
How each lot is tested
Every lot is tested in PrymaLab’s own laboratory in Ashland, Kentucky before release: RP-HPLC for purity, LC-MS for identity, Karl Fischer titration for water, LAL for endotoxin and USP <71> for sterility. The results for the lot now shipping are in the certificate panel on this page, and the certificate number, key and QR code can be checked on the verify page. These are in-house results from PrymaLab Analytical, not an independent test, and the lab is not accredited to ISO 17025. The third-party testing guarantee on this page still applies: if any independent laboratory finds the wrong compound, or less than the stated purity or amount, the order is refunded and the cost of the test is reimbursed.
Frequently asked questions
What is in GLOW 70 mg?
GHK-Cu 50 mg, BPC-157 10 mg and TB-500 10 mg, freeze-dried together in one vial. The TB-500 is full-length N-acetyl thymosin beta-4, 43 residues. The certificate for each lot confirms all three components by LC-MS and gives a purity result by RP-HPLC.
Is GLOW 70 mg just a bigger GLOW 50 mg?
No. GLOW 50 mg has its own ratio: 35 mg GHK-Cu, 5 mg BPC-157 and 10 mg TB-500. Relative to BPC-157 it carries twice as much thymosin beta-4 as this vial does. The two are separate products with separate certificates, not two fills of one formula.
Why does the blend look blue?
The copper(II) ion in GHK-Cu gives the cake, and any solution made from it, a blue colour. BPC-157 and thymosin beta-4 are white powders on their own, so the colour comes from the copper complex, which is most of the vial by mass.
Is there any research on GLOW itself?
No. The literature covers GHK-Cu, BPC-157 and thymosin beta-4 separately, mainly in cell cultures and rodents. No study has given the three together or measured how they behave as a mixture, so claims about the blend as a whole are extrapolation.
How should GLOW 70 mg be stored?
Keep the sealed vial at −20 °C, desiccated and protected from light, and allow it to reach room temperature before opening. Reconstituted solution belongs at 2 to 8 °C in the dark, used within a period your laboratory has set.
Is it for human use?
No. GLOW is sold for laboratory research only. It is not approved by the FDA or any other authority for human or veterinary use and must not be given to people or animals.
References
- Maquart FX, Pickart L, Laurent M, et al. Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+. FEBS Lett. 1988;238(2):343-6. DOI · PubMed
- Pickart L, Margolina A. Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data. Int J Mol Sci. 2018;19(7). DOI · PubMed
- Chang CH, Tsai WC, Lin MS, et al. The promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migration. J Appl Physiol (1985). 2011;110(3):774-80. DOI · PubMed
- Hsieh MJ, Liu HT, Wang CN, et al. Therapeutic potential of pro-angiogenic BPC157 is associated with VEGFR2 activation and up-regulation. J Mol Med (Berl). 2017;95(3):323-333. DOI · PubMed
- Huff T, Müller CS, Otto AM, et al. beta-Thymosins, small acidic peptides with multiple functions. Int J Biochem Cell Biol. 2001;33(3):205-20. DOI · PubMed
Research use only
For laboratory research use only. GLOW BLEND 70MG is not a drug, food, cosmetic or dietary supplement, is not approved by the FDA or any comparable authority for human or veterinary use, and must not be given to people or animals. It is sold to qualified researchers and institutions, and the buyer is responsible for lawful handling and disposal.
Reviews
There are no reviews yet.