Description
PrymaLab · Research use only
Preloaded Autoinjector | NAD+ | 3ml Pen | 100mg/ml
NAD+ at 100 mg/mL in bacteriostatic water, 300 mg in a 3 mL preloaded pen
NAD+ (nicotinamide adenine dinucleotide, oxidized) is a coenzyme, not a peptide; it is the dinucleotide that cells use to shuttle electrons and that sirtuins and PARPs break down as they work. This listing is the lower-strength NAD+ pen, a 3 mL cartridge of NAD+ in bacteriostatic water at 100 mg/mL, or 300 mg per pen, supplied ready-made. Research on NAD+ spans its separate pools inside the cell, its links to mitochondrial function, and the precursors studied to raise it.
Specifications
| Property | Value |
|---|---|
| Compound | NAD+ β-Nicotinamide adenine dinucleotide, oxidized (free acid); coenzyme, not a peptide |
| CAS number | 53-84-9 |
| Molecular formula | C21H27N7O14P2 |
| Theoretical mass | 663.4 Da |
| Sequence / structure | Dinucleotide: adenosine 5′-monophosphate joined through a pyrophosphate bridge to nicotinamide 5′-mononucleotide; oxidized form. |
| Contents | NAD+: 1500 mg per 3 mL pen; 300 mg per 3 mL pen (50% w/v) |
| Form | Sterile aqueous solution in bacteriostatic water (0.9% benzyl alcohol), supplied in a 3 mL glass cartridge inside a preloaded autoinjector pen fitted with a needle |
| Storage, sealed | Store at 2–8 °C in the original carton, protected from light |
| Safety data sheet | PL-SDS-115 |
What is in the pen
Each pen carries one 3 mL glass cartridge of sterile NAD+ solution. The NAD+ is the oxidized free acid, CAS 53-84-9, theoretical mass 663.4 Da, the same compound sold as powder in the vials. It is dissolved at 100 mg/mL, so a full cartridge holds 300 mg, which is about 0.45 mmol, or close to 151 mM. The solvent is bacteriostatic water containing 0.9% benzyl alcohol. According to the safety data sheet, benzyl alcohol is harmful if swallowed or inhaled, irritates the eyes and can cause allergic skin reactions, while NAD+ is not classified.
Refrigerate the pen at 2 to 8 °C in its original carton and away from light, keep it capped when not in use, and never freeze it. A solution that has frozen or turned cloudy or discoloured should be thrown away.
Pen or vial
PrymaLab sells NAD+ in five listings: the NAD+ 1000 mg vial and a 500 mg vial of dry powder, this pen, a stronger pen and a nasal spray. The vials keep the compound dry and frozen until the day it is dissolved, and the concentration is whatever the volume of diluent makes it. The pens give up that choice for a solution made in advance at a stated strength. At 100 mg/mL this pen is the more dilute of the two, a fifth of the strength of the NAD+ 500 mg/mL pen. The general case for each format is laid out in peptide pen vs vial.
What the research covers
NAD+ is not spread evenly through a cell. Cambronne and Kraus reviewed evidence in 2020 that steady-state NAD+ levels are compartmentalised within mammalian cells, and that cells regulate signalling by controlling how much NAD+ is made in each subcellular location1. On that view, a single NAD+ figure for a whole cell or tissue averages over pools that may be regulated separately1. Cantó, Menzies and Auwerx framed NAD+ metabolism as a balancing act between mitochondria and the nucleus, and described a cofactor that can rewire metabolism, activate sirtuins and maintain mitochondrial fitness through mechanisms that include the mitochondrial unfolded protein response2.
Much of the work on raising NAD+ in living systems has used precursors rather than the coenzyme itself3. Yoshino, Baur and Imai reviewed nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR) in 2018, reported that supplementing them had improved age-associated conditions in preclinical models, and noted that the fate of each depends on how a tissue expresses NAD+ biosynthetic enzymes, nucleotidases and presumed transporters3. They described the translation of those results to people as the next thing to test, not something already shown3.
None of these reviews studied NAD+ in a ready-made solution, none reported a controlled human trial of NAD+ itself, and none says how stable NAD+ is in water. For a pen, the storage limits on the safety data sheet are therefore the practical guide. The NAD+ guide covers the full literature, including the individual animal studies. None of the work used PrymaLab material.
How each lot is tested
Every lot is tested in PrymaLab’s own laboratory in Ashland, Kentucky before release: RP-HPLC for purity, LC-MS for identity, Karl Fischer titration for water, LAL for endotoxin and USP <71> for sterility. Published certificates are listed on the results page and can be checked by number and key on the verify page. The certificate covers the peptide lot used to fill the pen, not the finished solution. These are in-house results from PrymaLab Analytical, not an independent test, and the lab is not accredited to ISO 17025. The third-party testing guarantee on this page still applies: if any independent laboratory finds the wrong compound, or less than the stated purity or amount, the order is refunded and the cost of the test is reimbursed.
Frequently asked questions
What exactly is in the pen?
A 3 mL glass cartridge of oxidized NAD+ free acid dissolved at 100 mg/mL, 300 mg in total, in bacteriostatic water preserved with 0.9% benzyl alcohol. The pen arrives filled, so there is no powder to weigh or dissolve.
How does this pen compare with the 500 mg/mL pen?
Same compound and same solvent, different strength. This pen is at 100 mg/mL, 300 mg per cartridge; the stronger pen is at 500 mg/mL, 1,500 mg per cartridge. A study that needs the lower concentration can start from this one.
Does the certificate apply to the solution in the pen?
The certificate belongs to the NAD+ lot that went into the pen, which PrymaLab tests in its own laboratory. It does not report on the finished solution. Published certificates are on the results page and can be confirmed by number and key on the verify page.
How should the pen be stored?
Cold and dark: 2 to 8 °C in the original carton, capped when not in use. Freezing is not allowed, and a pen whose solution has frozen, clouded or changed colour should be discarded, as the safety data sheet states.
Can the pen be used in people or animals?
No. It is sold for laboratory research use only and is not approved by the FDA or any other regulator for human or veterinary use. It must not be given to people or animals.
References
- Cambronne XA, Kraus WL. Location, Location, Location: Compartmentalization of NAD(+) Synthesis and Functions in Mammalian Cells. Trends Biochem Sci. 2020;45(10):858-873. DOI · PubMed
- Cantó C, Menzies KJ, Auwerx J. NAD(+) Metabolism and the Control of Energy Homeostasis: A Balancing Act between Mitochondria and the Nucleus. Cell Metab. 2015;22(1):31-53. DOI · PubMed
- Yoshino J, Baur JA, Imai SI. NAD(+) Intermediates: The Biology and Therapeutic Potential of NMN and NR. Cell Metab. 2018;27(3):513-528. DOI · PubMed
Research use only
For laboratory research use only. This product is not a drug, food, cosmetic or dietary supplement, is not approved by the FDA or any comparable authority for human or veterinary use, and must not be given to people or animals. It is sold to qualified researchers and institutions, and the buyer is responsible for lawful handling and disposal.
Other formats of NAD+
NAD+ is also stocked as NAD+ 500mg and NAD+ Nasal Spray. Each listing states its own quantity and concentration, and the pen and vial comparison explains what changes between formats.
1 review for Preloaded Autoinjector | NAD+ | 3ml Pen | 100mg/ml