Description
PrymaLab · Research use only
DSIP 10mg Nasal Spray
DSIP in bacteriostatic water, 10 mg in a metered nasal spray bottle
DSIP is delta sleep-inducing peptide, the nonapeptide Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, named in 1977 for the EEG changes it produced in rabbits. Here it comes as a nasal spray, 10 mg of DSIP dissolved in bacteriostatic water with a preservative and packed in a metered spray bottle. Studies of DSIP itself delivered it into the brain’s ventricles or the bloodstream, while the nasal route has a literature of its own on whether molecules reach the brain from the nose.
Specifications
| Property | Value |
|---|---|
| Compound | DSIP Delta sleep-inducing peptide |
| CAS number | 62568-57-4 |
| Molecular formula | C35H48N10O15 |
| Theoretical mass | 848.8 Da |
| Sequence / structure | H-WAGGDASGE-OH (9 residues; free N- and C-termini) |
| Contents | DSIP: 10 mg per bottle (≤ 0.1% w/v) |
| Form | Sterile aqueous solution in bacteriostatic water (0.9% benzyl alcohol), supplied in a metered nasal spray bottle |
| Storage, sealed | Store at 2–8 °C, protected from light; do not freeze |
| Safety data sheet | PL-SDS-048 |
What is in the bottle
The bottle contains 10 mg of DSIP, about 11.8 µmol, dissolved in sterile bacteriostatic water that carries 0.9% benzyl alcohol to hold back microbial growth. According to the safety data sheet the peptide makes up no more than 0.1% w/v of the solution. It is the nonapeptide sold dry in the vials, H-WAGGDASGE-OH, theoretical mass 848.8 Da. Only the preservative has a hazard classification: benzyl alcohol is harmful by ingestion or inhalation, irritates the eyes and can sensitise skin, while DSIP itself is unclassified.
Refrigerate it at 2 to 8 °C, standing upright with the cap on and out of the light. Freezing is excluded. Why peptide solutions are kept cold and dark is explained in peptide storage and stability.
What the research covers
How DSIP was studied
The peptide was named after experiments in which infusion into the cerebral ventricles of rabbits enhanced delta and spindle EEG activity, an effect its synthetic fragments and analogues did not reproduce1. Later work found DSIP readily degraded in blood: incubated with human or rat blood it released tryptophan-like products, at rates that depended on temperature, time and species2. A peptide cleared that quickly in circulation is one reason a route that bypasses the blood draws interest.
The nasal route
A review by Lochhead and Thorne set out how the nose is built and by which routes a substance on its epithelium might reach the central nervous system while bypassing the blood-brain barrier, citing animal and human work on biologics3. That review concerns large molecules in general. DSIP is a small peptide, and none of the DSIP papers cited here used the nasal route.
What is not established
No reference on this page measured DSIP uptake from the nose, its stability in a preserved solution, or how much reaches the brain from a spray. The underlying biology is itself unsettled: no DSIP gene, precursor or receptor has been isolated. The DSIP research guide covers the full literature, and the overview of peptides studied for brain and cognition puts it in context.
How each lot is tested
Every lot is tested in PrymaLab’s own laboratory in Ashland, Kentucky before release: RP-HPLC for purity, LC-MS for identity, Karl Fischer titration for water, LAL for endotoxin and USP <71> for sterility. Published certificates are listed on the results page and can be checked by number and key on the verify page. The certificate covers the peptide lot used to fill the bottle, not the finished solution. These are in-house results from PrymaLab Analytical, not an independent test, and the lab is not accredited to ISO 17025. The third-party testing guarantee on this page still applies: if any independent laboratory finds the wrong compound, or less than the stated purity or amount, the order is refunded and the cost of the test is reimbursed.
Frequently asked questions
Is the spray the same DSIP as the vials?
Yes. Vials and spray contain the same nonapeptide, H-WAGGDASGE-OH, at 848.8 Da. In the vials it is a dry powder stored frozen at −20 °C; in the spray, 10 mg is already in bacteriostatic water and is refrigerated, not frozen.
How much DSIP is in a bottle?
Each bottle holds 10 mg, roughly 11.8 µmol, and the safety data sheet caps the concentration at 0.1% w/v. Studies that need to set their own concentration are better served by the 5 mg or 10 mg freeze-dried vials, which state a dry mass.
Has DSIP been studied by the nasal route?
Not in any paper cited on this page. The original rabbit experiments infused DSIP into the brain's ventricles, and the nasal delivery review covers biologics in general, so there are no findings specific to this format to point to.
What does the certificate cover for the spray?
The certificate belongs to the DSIP lot that went into the bottles; the finished solution has no certificate of its own. The testing section on this page describes what each certificate lists and where one can be verified.
Is the spray for human use?
No. DSIP is not a sleep aid, and this bottle is laboratory research material only. The FDA has not approved it for human or veterinary use, and it must not be given to people or animals.
References
- Schoenenberger GA, Monnier M. Characterization of a delta-electroencephalogram (-sleep)-inducing peptide. Proc Natl Acad Sci U S A. 1977;74(3):1282-6. DOI · PubMed
- Graf MV, Saegesser B, Schoenenberger GA. Degradation and aggregation of delta sleep-inducing peptide (DSIP) and two analogs in plasma and serum. Peptides. 1987;8(4):599-603. DOI · PubMed
- Lochhead JJ, Thorne RG. Intranasal delivery of biologics to the central nervous system. Adv Drug Deliv Rev. 2012;64(7):614-28. DOI · PubMed
Research use only
For laboratory research use only. DSIP 10mg Nasal Spray is not a drug, food, cosmetic or dietary supplement, is not approved by the FDA or any comparable authority for human or veterinary use, and must not be given to people or animals. It is sold to qualified researchers and institutions, and the buyer is responsible for lawful handling and disposal.
Other formats of DSIP
DSIP is also stocked as DSIP 5MG, DSIP 10MG and DSIP 10mg preloaded 3ml pen. Each listing states its own quantity and concentration, and the pen and vial comparison explains what changes between formats.
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