Description
PrymaLab · Research Use Only
DSIP 5mg
Delta sleep-inducing peptide · WAGGDASGE · CAS 62568-57-4
DSIP 5mg peptide is a lyophilized vial of delta sleep-inducing peptide, the nonapeptide Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, CAS 62568-57-4, molecular weight 848.82. It was first described in 1977 and no receptor for it has been identified in the five decades since.
Specification Table
| Property | Value |
|---|---|
| Compound | Delta sleep-inducing peptide |
| Vial content | 5 mg lyophilized powder |
| Molar content of vial | Approximately 5.89 µmol |
| Stock at 1 ml diluent | Approximately 5.89 mM |
| Common designation | DSIP |
| Alternative name | Emideltide |
| CAS number | 62568-57-4 |
| Molecular formula | C35H48N10O15 |
| Molecular weight | 848.82 g/mol |
| Residue count | 9 |
| Single-letter sequence | WAGGDASGE |
| Three-letter sequence | Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu |
| Net charge at neutral pH | Negative, from two acidic residues |
| Year first described | 1977 |
| Originating group | Schoenenberger and Monnier, University of Basel |
| Source of the original isolate | Cerebral venous blood of rabbits under electrical thalamic stimulation |
| Receptor | Not identified. No specific receptor has been characterised |
| Oxidation-prone residues | Tryptophan at position 1 |
| Deamidation-prone motifs | None. No Asn-Gly present |
| Appearance | White lyophilized powder |
| Purity | Per lot-specific certificate of analysis |
| Solubility | Water soluble |
| Storage, lyophilized | -20°C, protected from light and moisture |
| Storage, reconstituted | 2-8°C, protected from light |
| Regulatory status | No approved human or veterinary formulation in any jurisdiction |
How Was DSIP Discovered?
The discovery is unusual enough to be worth recounting, because it explains both the name and several of the difficulties that followed.
Schoenenberger and Monnier at the University of Basel were investigating whether a transferable factor mediated sleep states. They applied electrical stimulation to the thalamus of rabbits, collected cerebral venous blood, and fractionated it looking for activity in recipient animals.
The fraction they isolated and sequenced in 1977 was a nonapeptide, which they named for the electroencephalographic delta activity associated with it (Schoenenberger and Monnier, Proceedings of the National Academy of Sciences, 1977).
The approach was standard for its era and it produced a real, sequenced, synthesisable molecule. What it did not produce was a receptor, a gene, or a precursor protein, because the method worked backward from activity rather than forward from molecular biology.
That origin shapes everything downstream. A peptide identified by transferred activity carries the name of the activity, and the name then persists whether or not the mechanism is ever established.
What Does It Mean That No Receptor Has Been Found?
Nearly five decades of work has not identified a specific DSIP receptor, and this is the central fact about the compound.
For most bioactive peptides, receptor identification followed within years of sequencing, often through binding studies with radiolabelled ligand followed by expression cloning. That sequence of work was attempted for DSIP and did not converge on a receptor.
Several possibilities remain open. The peptide may act through a low-affinity interaction that binding assays struggle to resolve, and it may act indirectly, through effects on another system rather than through a dedicated receptor. Or the original activity may have been attributable to something else in the fraction.
No precursor protein or gene encoding the DSIP sequence has been identified in any genome either, which is a further difficulty, since endogenous peptides are normally encoded within a precursor, and the absence of one raises questions about whether the sequence occurs naturally at all.
Graf and Kastin reviewed the position in the 1980s and Kovalzon and Strekalova revisited it in 2006, and the receptor question remained open in both.
What Is Known About the Chemistry?
Setting the biology of DSIP 5mg peptide aside, the molecule itself is straightforward and its properties are fully determined.
Nine residues at 848.82 daltons, with a sequence dominated by small residues. Four of the nine are glycine or alanine, which gives the chain considerable conformational freedom and makes stable secondary structure unlikely at this length.
Two acidic residues, aspartate at position 5 and glutamate at position 9, give a net negative charge at neutral pH, and that aids aqueous solubility and reduces adsorption to negatively charged surfaces relative to a basic peptide.
The single tryptophan at position 1 is the only oxidation-sensitive residue and the only significant chromophore. It provides absorbance at 280 nm useful for concentration determination, and it is the residue to watch for a plus-16 dalton satellite in mass spectrometry.
No cysteine means no disulfide chemistry. No asparagine-glycine motif means the principal deamidation route is absent. Chemically this is a forgiving peptide, which contrasts sharply with how unsettled its biology remains.
What Would Rigorous DSIP 5mg Peptide Work Require?
Given the unresolved mechanism behind DSIP 5mg peptide, the most defensible designs are those that do not presuppose one.
A scrambled-sequence control is more important here than for a compound with a known target. Without a receptor, there is no binding assay to demonstrate specificity, so a composition-matched randomised sequence becomes the primary specificity control available.
Concentration-response across a wide range matters, because no receptor affinity is known and therefore no concentration can be justified from first principles. Single-concentration designs in this area rest on values inherited from earlier work without independent justification.
Where an effect is observed, the immediate follow-up question is whether it is peptide-specific or attributable to degradation products, since a nonapeptide breaks down into fragments and free amino acids that have their own activities.
Independent replication carries unusual weight for this compound. A literature spanning five decades without receptor identification suggests either a subtle mechanism or an unreliable phenomenon, and distinguishing between those requires reproducibility work rather than novel extension.
How Should the Name Be Read?
This compound carries a name that states an activity, which is unusual and which creates a reading problem worth addressing directly.
Peptides are typically named for their sequence, their source, their discoverer or an arbitrary code. Names encoding a claimed function are rarer, and they persist unchanged even when the function becomes contested, because renaming an entity in the literature is impractical once citations accumulate.
Delta sleep-inducing peptide was named in 1977 on the basis of the transferred activity observed in the original rabbit experiments. The name records what the discoverers believed they had found at that time.
Nearly five decades later, without an identified receptor, without a precursor gene, and with a declining publication record, the name carries more assertion than the evidence supports. It is a historical label rather than a description.
The alternative designation emideltide exists partly for this reason, offering a neutral name without an embedded claim, though it is used far less often in practice.
For anyone writing about the compound, using the acronym or the alternative name avoids restating a claim in every mention, and that is a small precision that matters more for this compound than for almost any other in the catalogue.
What Does the Sequence Suggest About Stability In Situ?
Beyond storage stability, the sequence has features that bear on how long the peptide persists in a biological system, which is a separate question.
The amino terminus carries tryptophan, an aromatic residue. Aminopeptidases show varying preferences and bulky aromatic residues at the amino terminus are not universally favoured, though this offers less protection than a proline or a D-amino acid would.
The carboxy terminus is glutamate, a free acid rather than an amide. Carboxypeptidases act at this terminus, and an unmodified acidic terminus offers no particular resistance.
Internally, the glycine-rich central region is conformationally flexible and therefore accessible to endopeptidases, since proteases generally require their substrate to adopt an extended conformation in the active site and flexible regions accommodate that readily.
Taken together the sequence carries no obvious protective features, which suggests limited persistence in any system with peptidase activity. That is worth accounting for in the design rather than discovering afterward.
A peptidase inhibitor cocktail, or a defined time course rather than a single endpoint, addresses it depending on what the question requires.
What Does DSIP 5mg Peptide Cost in Molar Terms?
Molar accounting for a nonapeptide is unusually favourable, and it is worth doing before planning a series.
At 848.82 daltons, DSIP 5mg peptide supplies approximately 5.89 micromoles. Compare that to five milligrams of a 4000-dalton construct, which yields barely a fifth as much, and the practical difference in how many conditions a single vial covers becomes obvious.
A full concentration series spanning six orders of magnitude, in triplicate, with scrambled-sequence and vehicle arms alongside, remains comfortably within one vial at typical cell-culture volumes.
That matters here more than for most compounds. Without a known receptor affinity there is no basis for narrowing the range, so wide ranging is a requirement rather than a luxury.
Counterion correction still applies, so ask for net peptide content.
Reconstitution and Storage in Laboratory Practice
Five milligrams is approximately 5.89 micromoles, and reconstituted into 1 ml that gives roughly a 5.89 mM stock.
Water solubility is good, helped by the two acidic residues. Add diluent slowly down the vial wall and swirl gently rather than shaking.
Protect from light on account of the tryptophan, which is both photosensitive and oxidation-prone. Minimise headspace air exposure and avoid leaving the vial open. With only one oxidisable residue the risk is lower than for tryptophan-rich sequences, but it is the one degradation route worth managing.
The dry powder keeps at -20°C, sealed, dark and dry. In solution it keeps at 2-8°C in the dark, split into aliquots ahead of any freeze. Record lot, diluent, volume, concentration and date.
One further practical note concerns nomenclature in ordering. Material may be listed under the acronym, under the full descriptive name, or under emideltide, and all three refer to the same nonapeptide.
Confirming the sequence rather than the name avoids the ambiguity entirely. WAGGDASGE is unambiguous in a way that any of the three names is not.
That check costs nothing and it forecloses a category of ordering error that recurs with compounds carrying multiple designations.
Published Literature
All references verified against the publisher record. Note that the most recent substantive review dates from 2006, which itself indicates the state of activity in this area.
- Schoenenberger GA, Monnier M. Proceedings of the National Academy of Sciences. 1977;74(3):1282-1286.
- Graf MV, Kastin AJ. Neuroscience and Biobehavioral Reviews. 1984;8(1):83-93.
- Kovalzon VM, Strekalova TV. Neuroscience and Biobehavioral Reviews. 2006;30(1):1-7.
- Graf MV, Kastin AJ. Peptides. 1986;7(6):1165-1187.
- Iyer KS, McCann SM. Neuroendocrinology. 1987;46(1):93-95.
Frequently Asked Questions
What is DSIP 5mg peptide?
A lyophilized vial of delta sleep-inducing peptide, the nonapeptide Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, CAS 62568-57-4, molecular weight 848.82. Five milligrams is approximately 5.89 micromoles. It is supplied for laboratory research alone, with no human or veterinary approval anywhere.
How was DSIP discovered?
Schoenenberger and Monnier at Basel applied electrical stimulation to the thalamus of rabbits, collected cerebral venous blood, and fractionated it looking for transferable activity. The fraction they sequenced in 1977 was this nonapeptide, named for the associated electroencephalographic delta activity.
Why does the discovery method matter?
Because it worked backward from activity rather than forward from molecular biology. That produced a real sequenced molecule but no receptor, gene or precursor protein, and a peptide named for an activity keeps that name whether or not a mechanism is later established.
Has a DSIP receptor been identified?
No. Nearly five decades of work has not identified one, which is the central fact about this compound. For most bioactive peptides, receptor identification followed within years of sequencing through radioligand binding and expression cloning. That sequence did not converge here.
What explanations remain open?
Several. The peptide may act through a low-affinity interaction that binding assays struggle to resolve, or indirectly through another system rather than a dedicated receptor. Or the original transferred activity may have been attributable to something else in the fraction.
Is there a gene encoding DSIP?
No precursor protein or gene encoding this sequence has been identified in any genome. Endogenous peptides are normally encoded within a precursor, so the absence raises a question about whether the sequence occurs naturally at all.
What are the chemical properties?
Nine residues at 848.82 daltons, with four glycine or alanine giving considerable conformational freedom, and two acidic residues giving a net negative charge at neutral pH that aids solubility. One tryptophan provides the only chromophore and the only oxidation-sensitive site.
Is DSIP chemically stable?
Reasonably. No cysteine means no disulfide chemistry, and no asparagine-glycine motif means the principal deamidation route is absent. The single tryptophan is the only real concern, which contrasts sharply with how unsettled the biology remains.
What controls does this compound require?
A scrambled-sequence control above all, since without a receptor there is no binding assay to demonstrate specificity and a composition-matched randomised sequence becomes the primary control available. Wide concentration-response is also necessary since no affinity is known.
What would advance the field?
Independent replication rather than extension. Five decades without receptor identification suggests either a subtle mechanism or an unreliable phenomenon, and distinguishing between those requires reproducibility work more than it requires novel observations.
Compliance Statement
DSIP 5mg peptide is sold exclusively for laboratory research use. It is not a drug, food, or cosmetic product, and it is not a dietary product of any kind. It is not approved by the FDA or any comparable authority for human or veterinary use, and no receptor for this compound has been identified in nearly five decades of published work. This product is not intended to diagnose, treat, cure, or prevent any disease. It must not be given to humans or animals. Purchase is restricted to qualified researchers and institutions operating within applicable laws. All handling is the responsibility of the purchasing laboratory.
Other formats of DSIP
DSIP is also stocked as DSIP 10mg Nasal Spray, DSIP 10mg preloaded 3ml pen and DSIP 10MG. Each listing states its own quantity and concentration, and the pen and vial comparison explains what changes between formats.
19 reviews for DSIP 5MG