Description
PrymaLab · Research use only
Bronchogen 20mg Nasal Spray
Bronchogen tetrapeptide (AEDL), 20 mg as a sterile solution in a metered spray bottle
Bronchogen nasal spray contains Ala-Glu-Asp-Leu, the 446.5 Da tetrapeptide that the Khavinson series links to the bronchi and lungs. The bottle holds 20 mg of Bronchogen as a sterile solution in bacteriostatic water, at no more than 0.2% w/v by the safety data sheet. Research on the peptide covers bronchial cell cultures, DNA melting and docking models. A separate literature on nasal drug delivery deals with peptides in general and has not examined this one.
Specifications
| Property | Value |
|---|---|
| Compound | Bronchogen Ala-Glu-Asp-Leu (AEDL); Khavinson bronchial peptide bioregulator |
| CAS number | Not assigned |
| Molecular formula | C18H30N4O9 |
| Theoretical mass | 446.5 Da |
| Sequence / structure | H-AEDL-OH (4 residues; free N- and C-termini) |
| Contents | Bronchogen: 20 mg per bottle (≤ 0.2% w/v) |
| Form | Sterile aqueous solution in bacteriostatic water (0.9% benzyl alcohol), supplied in a metered nasal spray bottle |
| Storage, sealed | Store at 2–8 °C, protected from light; do not freeze |
| Safety data sheet | PL-SDS-025 |
What is in the bottle
Each bottle carries 20 mg of AEDL, the tetrapeptide also offered as powder in the Bronchogen 20 mg vial. Here it is dissolved in bacteriostatic water, a 0.9% benzyl alcohol solution, with the peptide making up 0.2% w/v or less. The peptide is not classified on the safety data sheet. The preservative is: benzyl alcohol is harmful if swallowed or inhaled, irritates the eyes and can sensitise skin.
Stand the bottle upright with its cap on, in a refrigerator at 2 to 8 °C and out of the light, and never let it freeze. The peptide storage and stability article covers peptide solutions more generally.
What the research covers
Bronchogen’s own research is cellular and computational. In cultures of human embryonic bronchial cells, it raised expression of the differentiation factors CXCL12 and Hoxa3 in the epithelial cells, and the increase was greater in late-passage cultures1. Docking models from Khavinson’s group put AEDL on the DNA sequence ctcc, a prediction rather than a measured binding2. None of this work involved the nose, and the bronchial cells were grown in dishes, not reached through an airway.
Interest in spraying peptides into the nose belongs to the drug delivery field. Writing in 2002, Illum surveyed the nasal formulations that companies were then building for generic drugs and peptides, and reviewed the discovery of a direct nose-to-brain transport route through the olfactory region, with early proof-of-concept examples3. It does not discuss Bronchogen or any Khavinson peptide, and nothing in these references measured how AEDL crosses the nasal lining or where it goes afterwards.
There are no animal or human studies of Bronchogen among these references. The Bronchogen guide covers the full literature on the peptide.
How each lot is tested
Every lot is tested in PrymaLab’s own laboratory in Ashland, Kentucky before release: RP-HPLC for purity, LC-MS for identity, Karl Fischer titration for water, LAL for endotoxin and USP <71> for sterility. Published certificates are listed on the results page and can be checked by number and key on the verify page. The certificate covers the peptide lot used to fill the bottle, not the finished solution. These are in-house results from PrymaLab Analytical, not an independent test, and the lab is not accredited to ISO 17025. The third-party testing guarantee on this page still applies: if any independent laboratory finds the wrong compound, or less than the stated purity or amount, the order is refunded and the cost of the test is reimbursed.
Frequently asked questions
Is the spray the same Bronchogen as the vial?
Yes. Ala-Glu-Asp-Leu is the peptide in every Bronchogen listing, at 20 mg each. What differs is the form: dry, freeze-dried powder in the vial, kept at −20 °C, against a sterile bacteriostatic water solution in the bottle, kept refrigerated and never frozen.
What is the solution made of?
Water, benzyl alcohol at 0.9% as the preservative, and 20 mg of Bronchogen, which the safety data sheet puts at 0.2% w/v or below. Nothing else is listed on the sheet, and the peptide amount is printed on the bottle label.
Has Bronchogen been studied by the nasal route?
Not in the references checked for this page. The Bronchogen studies used cell cultures, purified DNA and computer models, and the nasal delivery review cited here concerns drugs and peptides in general without mentioning Bronchogen.
Does the spray hold AEDL or ADEL?
AEDL, Ala-Glu-Asp-Leu, as stated on the certificate for the peptide lot used to fill it. Papers on bronchogen do not all agree on the order of the glutamic and aspartic acid residues, and because both orders weigh the same, check the sequence named in any study you compare against.
Is Bronchogen nasal spray intended for people?
No. PrymaLab supplies the spray to laboratories as a research material. Neither the FDA nor any other regulator has approved Bronchogen for human or veterinary use, so it is never to be sprayed into, or otherwise given to, a person or an animal.
References
- Khavinson VKh, Linkova NS, Polyakova VO, et al. Peptides tissue-specifically stimulate cell differentiation during their aging. Bull Exp Biol Med. 2012;153(1):148-51. DOI · PubMed
- Khavinson VK, Lin'kova NS, Tarnovskaya SI. Short Peptides Regulate Gene Expression. Bull Exp Biol Med. 2016;162(2):288-292. DOI · PubMed
- Illum L. Nasal drug delivery: new developments and strategies. Drug Discov Today. 2002;7(23):1184-9. DOI · PubMed
Research use only
For laboratory research use only. Bronchogen 20mg Nasal Spray is not a drug, food, cosmetic or dietary supplement, is not approved by the FDA or any comparable authority for human or veterinary use, and must not be given to people or animals. It is sold to qualified researchers and institutions, and the buyer is responsible for lawful handling and disposal.
Other formats of Bronchogen
Bronchogen is also stocked as Bronchogen 20mg preloaded 3ml pen. Each listing states its own quantity and concentration, and the pen and vial comparison explains what changes between formats.
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