Description
PrymaLab · Research use only
Bronchogen Peptide 20mg
Ala-Glu-Asp-Leu (AEDL), the Khavinson bronchial tetrapeptide, in a 20 mg vial
Bronchogen is the synthetic tetrapeptide Ala-Glu-Asp-Leu (AEDL), the peptide that Vladimir Khavinson’s bioregulator series assigns to the bronchi and lungs. This listing is a 20 mg vial of freeze-dried Bronchogen with a theoretical mass of 446.5 Da. Its research record is cell-culture and biophysical work from one network of authors: differentiation factors in human bronchial epithelial cultures, DNA melting measurements, and docking models of DNA and transporter binding.
Specifications
| Property | Value |
|---|---|
| Compound | Bronchogen Ala-Glu-Asp-Leu (AEDL); Khavinson bronchial peptide bioregulator |
| CAS number | Not assigned |
| Molecular formula | C18H30N4O9 |
| Theoretical mass | 446.5 Da |
| Sequence / structure | H-AEDL-OH (4 residues; free N- and C-termini) |
| Label claim | 20 mg/vial |
| Form | Lyophilized (freeze-dried) powder or cake in a sealed glass vial with a rubber stopper and aluminium crimp cap |
| Storage, sealed | −20 °C, desiccated, dark |
| Purity specification | 99.0% or higher by RP-HPLC |
| Lot certificate | Results for the lot now shipping are in the certificate panel on this page |
| Safety data sheet | PL-SDS-023 |
Which Bronchogen sequence this vial contains
The lot certificate gives the sequence as H-AEDL-OH, formula C18H30N4O9, theoretical mass 446.5 Da. In molar terms 20 mg is about 45 µmol, a little less after residual water. The literature is not consistent about the order of the two acidic residues. Several papers write bronchogen as Ala-Glu-Asp-Leu1, but the 2011 DNA-melting study by Monaselidze, Khavinson and colleagues gives it as Ala-Asp-Glu-Leu in its title2. The two orders contain the same four amino acids, so they share formula and mass, and an LC-MS identity result confirms the composition without telling them apart. The certificate for this vial states the AEDL order. When comparing results with a paper, check which order its authors used.
What the research covers
What Bronchogen is
Bronchogen is one of the short synthetic peptides from Khavinson’s programme, which Anisimov and Khavinson describe as starting with small peptides isolated from organs and tissues and moving on to synthetic di-, tri- and tetrapeptide analogues3. The group’s model holds that peptides of two to seven residues enter the nucleus, bind histones and DNA, and regulate gene expression and DNA methylation4. For AEDL there is both a docking prediction and a physical measurement. Docking models from 2016 placed AEDL, along with the tripeptide EDL, on the DNA sequence ctcc5.
DNA melting
Monaselidze and colleagues measured DNA melting in the presence of bronchogen by differential scanning microcalorimetry2. The peptide raised the melting temperature of calf thymus and mouse liver DNA by 3.1 °C over a narrow range of peptide-to-base-pair ratios, with no further change at higher ratios, while the melting enthalpy stayed the same. The authors concluded that bronchogen stabilises DNA, is neither adenine-thymine nor guanine-cytosine specific, and binds both strands, mainly at the bases2. That result describes isolated DNA in solution. It does not show binding inside cells.
Bronchial epithelial cultures
In a 2012 study, Khavinson, Linkova, Polyakova and colleagues measured the differentiation factors CXCL12 and Hoxa3 in human embryonic bronchial cell cultures and found both reduced in late-passage, aging cultures1. Bronchogen stimulated their expression in bronchial epithelial cells, and the effect was larger in the aged cultures. Run in parallel, pancragen raised the same markers in pancreatic cells and vesugen raised CXCL12 and WEGC1 in prostatic fibroblasts, which the authors presented as evidence of tissue specificity1.
A 2023 docking study listed AEDL among 26 active ultrashort peptides that scored higher for binding to the membrane transporters LAT1, LAT2 and PEPT1 than di- and tripeptides with no known activity6. Like the 2016 DNA model, it is a computed prediction.
What is not established
None of these references reports Bronchogen in lung tissue, in a living animal or in people. The bronchial findings come from embryonic cell cultures, the DNA work from purified DNA, and the rest from computer models. All of the papers share authors with Khavinson’s group, and most were published in Bulletin of Experimental Biology and Medicine, the English edition of a Russian journal. The Bronchogen guide covers the programme’s wider claims, which reach much further than these abstracts.
How each lot is tested
Every lot is tested in PrymaLab’s own laboratory in Ashland, Kentucky before release: RP-HPLC for purity, LC-MS for identity, Karl Fischer titration for water, LAL for endotoxin and USP <71> for sterility. The results for the lot now shipping are in the certificate panel on this page, and the certificate number, key and QR code can be checked on the verify page. These are in-house results from PrymaLab Analytical, not an independent test, and the lab is not accredited to ISO 17025. The third-party testing guarantee on this page still applies: if any independent laboratory finds the wrong compound, or less than the stated purity or amount, the order is refunded and the cost of the test is reimbursed.
Frequently asked questions
Is Bronchogen Ala-Glu-Asp-Leu or Ala-Asp-Glu-Leu?
This vial is Ala-Glu-Asp-Leu (AEDL), as the certificate states. Some papers write the sequence with the two acidic residues swapped. Both orders have the same mass, so when comparing with a study, check which sequence the authors actually used.
How is Bronchogen different from Chonluten?
Both are assigned to the airways in the Khavinson series, but they are different molecules. Bronchogen is the tetrapeptide Ala-Glu-Asp-Leu; Chonluten is the tripeptide Glu-Asp-Gly. Their studies are separate, and results for one should not be read across to the other.
How do I check the certificate for my vial?
Look for the lot number printed on the vial. The certificate panel on this page shows the lot currently shipping, the testing results page lists earlier ones, and the verify page checks a certificate by number and key.
How should Bronchogen be stored?
Sealed, at −20 °C, desiccated and in the dark. Let the vial reach room temperature before you open it. The safety data sheet puts reconstituted solutions at 2 to 8 °C, protected from light, for a period set by your laboratory.
What has Bronchogen been shown to do?
In human embryonic bronchial cell cultures it raised two differentiation factors, more so in aging cultures, and in a test tube it raised the melting temperature of DNA. Everything else is computer modelling. There are no lung tissue, animal or human results among these references.
Can Bronchogen be used in people or animals?
No. It is a laboratory research material, not a drug or dietary supplement, and no regulator has approved it for human or veterinary use. It must not be given to people or animals.
References
- Khavinson VKh, Linkova NS, Polyakova VO, et al. Peptides tissue-specifically stimulate cell differentiation during their aging. Bull Exp Biol Med. 2012;153(1):148-51. DOI · PubMed
- Monaselidze JR, Khavinson VKh, Gorgoshidze MZ, et al. Effect of the peptide bronchogen (Ala-Asp-Glu-Leu) on DNA thermostability. Bull Exp Biol Med. 2011;150(3):375-7. DOI · PubMed
- Anisimov VN, Khavinson VKh. Peptide bioregulation of aging: results and prospects. Biogerontology. 2010;11(2):139-49. DOI · PubMed
- Khavinson VK, Popovich IG, Linkova NS, et al. Peptide Regulation of Gene Expression: A Systematic Review. Molecules. 2021;26(22). DOI · PubMed
- Khavinson VK, Lin'kova NS, Tarnovskaya SI. Short Peptides Regulate Gene Expression. Bull Exp Biol Med. 2016;162(2):288-292. DOI · PubMed
- Khavinson VK, Linkova NS, Rudskoy AI, et al. Feasibility of Transport of 26 Biologically Active Ultrashort Peptides via LAT and PEPT Family Transporters. Biomolecules. 2023;13(3). DOI · PubMed
Research use only
For laboratory research use only. Bronchogen Peptide 20mg is not a drug, food, cosmetic or dietary supplement, is not approved by the FDA or any comparable authority for human or veterinary use, and must not be given to people or animals. It is sold to qualified researchers and institutions, and the buyer is responsible for lawful handling and disposal.
Other formats of Bronchogen
Bronchogen is also stocked as Bronchogen 20mg Nasal Spray and Bronchogen 20mg preloaded 3ml pen. Each listing states its own quantity and concentration, and the pen and vial comparison explains what changes between formats.
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