BAC Water (Bacteriostatic Water) 3ML

What is Bacteriostatic Water for Peptides?

Bacteriostatic water peptides solution represents the gold standard for peptide reconstitution in research and pharmaceutical applications. PrymaLab’s bacteriostatic water for injection contains pharmaceutical-grade sterile water combined with 0.9% benzyl alcohol as a bacteriostatic preservative. This precise formulation of bac water for peptides enables researchers to safely reconstitute lyophilized peptides while maintaining solution sterility for up to 28 days after opening. Unlike sterile water which must be discarded after single use, bacteriostatic water provides cost-effective multi-dose capability essential for extended research protocols.

The bacteriostatic water mechanism relies on benzyl alcohol’s antimicrobial properties to inhibit bacterial growth without killing existing microorganisms. This bacteriostatic action creates an environment where bacteria cannot reproduce, effectively preventing contamination during repeated withdrawals from the same vial. Each 3ML vial of bac water maintains a carefully controlled pH range between 4.5 and 7.0, with an average of 5.7, ensuring optimal compatibility with sensitive peptide compounds. The pharmaceutical-grade rubber stopper allows for multiple needle penetrations while maintaining the sterile barrier that protects your valuable research materials.

Researchers choose bacteriostatic water for peptides because it offers unmatched convenience and reliability for reconstitution protocols. The 3ML volume provides sufficient bac water for multiple peptide vials while remaining compact for efficient storage. Whether reconstituting growth hormone peptides, healing peptides like BPC-157, or metabolic peptides such as semaglutide, bacteriostatic water for injection delivers consistent results. The benzyl alcohol preservative ensures that each withdrawal from day one through day 28 maintains the same sterile quality, eliminating concerns about bacterial contamination that plague sterile water after opening.

Composition and Pharmaceutical Standards

PrymaLab’s bacteriostatic water peptides formulation adheres to strict USP (United States Pharmacopeia) standards for pharmaceutical-grade reconstitution solutions. Each milliliter contains precisely 0.9% benzyl alcohol (9mg/mL) dissolved in sterile water for injection. This concentration represents the optimal balance between effective bacteriostatic action and minimal tissue irritation. The bac water undergoes rigorous sterility testing, endotoxin analysis, and pH verification before packaging to ensure compliance with pharmaceutical manufacturing standards.

The sterile water base used in bacteriostatic water for injection originates from highly purified water that meets or exceeds USP specifications for Water for Injection (WFI). Multiple distillation and filtration stages remove all particulates, pyrogens, and microbial contaminants. The addition of pharmaceutical-grade benzyl alcohol occurs under strictly controlled aseptic conditions in ISO-certified clean rooms. Each batch of bac water for peptides receives comprehensive quality control testing including sterility assurance, particulate matter analysis, and chemical composition verification.

The 3ML vial design incorporates pharmaceutical-grade Type I borosilicate glass that prevents chemical interactions with the bacteriostatic water contents. The rubber stopper consists of specially formulated elastomer that maintains integrity through multiple needle penetrations while preventing bacterial ingress. An aluminum crimp seal secures the stopper and provides tamper-evident protection. This packaging system ensures that bacteriostatic water peptides solution maintains its sterile, preservative-protected state from manufacturing through the entire 28-day usage period after opening.

How Bacteriostatic Water Works

The bacteriostatic mechanism of bac water relies on benzyl alcohol’s unique antimicrobial properties that inhibit bacterial reproduction without causing immediate cell death. When bacteria encounter the 0.9% benzyl alcohol concentration in bacteriostatic water for peptides, the alcohol molecules disrupt bacterial cell membrane integrity and interfere with essential metabolic processes. This disruption prevents bacteria from dividing and multiplying, effectively maintaining a static bacterial population that cannot establish infection or contamination. Unlike bactericidal agents that kill bacteria outright, the bacteriostatic action of bac water creates a hostile environment for growth while allowing any existing organisms to remain dormant.

The benzyl alcohol in bacteriostatic water for injection penetrates bacterial cell walls and interferes with protein synthesis mechanisms essential for reproduction. This interference occurs at multiple cellular levels, affecting ribosomal function, enzyme activity, and membrane transport systems. The cumulative effect prevents bacterial cells from completing their reproductive cycle, maintaining the sterile integrity of reconstituted peptide solutions. The 0.9% concentration provides sufficient antimicrobial activity to protect against common laboratory contaminants while remaining safe for injection when properly diluted with peptide compounds.

Bacteriostatic water peptides solutions maintain their protective properties throughout the 28-day usage period because benzyl alcohol remains stable and active in aqueous solution. The preservative does not degrade significantly under proper storage conditions, ensuring consistent antimicrobial protection from first use to final withdrawal. This sustained activity distinguishes bac water from sterile water, which offers no protection against contamination after the seal breaks. Researchers can confidently perform multiple reconstitutions from a single bacteriostatic water vial, knowing that each withdrawal receives the same level of contamination protection.

Bacteriostatic Water vs Sterile Water

Understanding the critical differences between bacteriostatic water for peptides and sterile water helps researchers select the appropriate reconstitution solution for their protocols. Sterile water contains only purified water that has undergone sterilization to eliminate all viable microorganisms. Once opened, sterile water vials must be used immediately and discarded, as they contain no preservative to prevent bacterial growth. In contrast, bacteriostatic water for injection contains 0.9% benzyl alcohol preservative that inhibits bacterial reproduction, enabling safe multi-dose use over 28 days. This fundamental difference makes bac water the preferred choice for research protocols requiring multiple peptide reconstitutions.

The cost-effectiveness of bacteriostatic water versus sterile water becomes apparent in extended research projects. A single 3ML vial of bac water for peptides can reconstitute multiple peptide vials over nearly a month, while sterile water requires a fresh vial for each reconstitution. This multi-dose capability reduces both material costs and storage requirements. Researchers working with expensive peptide compounds particularly value bacteriostatic water’s ability to maintain sterility between uses, eliminating waste from partially used sterile water vials that must be discarded.

Safety profiles differ significantly between bacteriostatic water peptides solutions and sterile water. The benzyl alcohol in bac water provides continuous antimicrobial protection that guards against contamination from repeated needle insertions. Sterile water offers no such protection, making it vulnerable to bacterial introduction with each vial access. However, bacteriostatic water for injection should not be used in neonates or infants due to benzyl alcohol toxicity concerns in this population. For adult research applications and peptide reconstitution, bac water delivers superior contamination protection and practical convenience compared to sterile water’s single-use limitation.

PEPTIDE RECONSTITUTION PROTOCOLS

Step-by-Step Bacteriostatic Water Reconstitution Guide

Proper peptide reconstitution using bacteriostatic water for peptides requires meticulous attention to sterile technique and precise volume measurements. Begin by assembling all necessary materials in a clean workspace: your lyophilized peptide vial, bacteriostatic water for injection, sterile insulin syringes (typically 1mL with 100 unit markings), alcohol prep pads, and a peptide calculator for dosing verification. Allow refrigerated bac water to reach room temperature before use, as cold solution can cause peptide precipitation. This preparation ensures optimal reconstitution conditions for your valuable peptide compounds.

The reconstitution process starts with thorough hand washing and workspace disinfection. Remove the plastic caps from both the peptide vial and bacteriostatic water vial, then swab both rubber stoppers with alcohol prep pads and allow them to air dry completely. Draw the calculated volume of bac water for peptides into your sterile syringe, being careful to eliminate any air bubbles that could affect measurement accuracy. Insert the needle through the peptide vial’s rubber stopper at a slight angle, directing the bacteriostatic water stream against the inside wall of the vial rather than directly onto the lyophilized powder. This gentle technique prevents peptide degradation from forceful impact.

After injecting the bacteriostatic water for injection into the peptide vial, remove the syringe and allow the solution to sit undisturbed for several minutes. The bac water will gradually dissolve the lyophilized peptide without agitation. Avoid shaking or vigorous swirling, as these actions can denature sensitive peptide bonds. Instead, gently roll the vial between your palms if needed to encourage complete dissolution. The reconstituted peptide solution should appear clear and free of particulates. Once fully dissolved, your peptide is ready for use, and the bacteriostatic water preservative will maintain sterility for subsequent withdrawals over the next 28 days.

Calculating Bacteriostatic Water Volumes

Accurate bacteriostatic water peptides volume calculations ensure precise dosing and optimal peptide concentration. The fundamental formula divides the total peptide amount (in milligrams) by the desired concentration (in mg/mL) to determine the required bac water volume. For example, reconstituting a 5mg peptide vial to achieve a 1mg/mL concentration requires 5mL of bacteriostatic water for injection. However, practical considerations often favor higher concentrations to minimize injection volumes and preserve peptide stability.

Common peptide reconstitution ratios using bacteriostatic water for peptides include 2mg peptide with 2mL bac water (1mg/mL), 5mg peptide with 2.5mL bac water (2mg/mL), and 10mg peptide with 2mL bac water (5mg/mL). These concentrations balance ease of measurement with practical injection volumes. A bac water calculator simplifies these computations, allowing researchers to input their peptide amount and desired dose to receive exact reconstitution volumes and corresponding syringe measurements. Most insulin syringes display 100 units per mL, so a 0.1mL dose equals 10 units on the syringe scale.

The 3ML volume of PrymaLab’s bacteriostatic water vial provides sufficient capacity for multiple peptide reconstitutions while maintaining compact storage. Researchers can reconstitute one large peptide vial (10mg with 2-3mL bac water) or multiple smaller vials (three 5mg vials with 1mL each). The multi-dose capability of bacteriostatic water for injection means a single 3ML vial serves extended research protocols without waste. Always document your reconstitution date and concentration on the peptide vial label to ensure accurate dosing throughout the 28-day usage period.

Common Peptide Applications

Bacteriostatic water for peptides serves as the universal reconstitution solution for diverse peptide classes used in research applications. Growth hormone releasing peptides (GHRPs) including GHRP-2, GHRP-6, and Ipamorelin require bac water for proper dissolution and multi-dose storage. These peptides typically come in 5mg vials and reconstitute well with 2-2.5mL of bacteriostatic water for injection, creating concentrations suitable for precise microgram dosing. The bacteriostatic preservative maintains peptide stability between doses, essential for protocols spanning several weeks.

Healing and recovery peptides like BPC-157 and TB-500 benefit significantly from bacteriostatic water peptides reconstitution. BPC-157 commonly arrives in 5mg vials that researchers reconstitute with 2.5mL bac water, yielding a 2mg/mL concentration ideal for the typical 250-500mcg research doses. TB-500, often supplied in 5mg vials, reconstitutes effectively with 2mL bacteriostatic water for injection to create a 2.5mg/mL solution. The 28-day shelf life of bac water perfectly accommodates the extended administration schedules these healing peptides require for optimal tissue repair research.

Metabolic peptides including semaglutide, tirzepatide, and other GLP-1 analogs require careful reconstitution with bacteriostatic water for peptides to maintain their complex molecular structures. These peptides often come in larger vial sizes (5-10mg) and benefit from the precise pH control that bac water provides. Researchers reconstituting semaglutide typically use 2-3mL of bacteriostatic water for injection per 5mg vial, creating concentrations that allow accurate measurement of the small doses these potent compounds require. The benzyl alcohol preservative in bac water ensures these expensive peptides remain uncontaminated throughout multi-week research protocols.

Reconstitution Best Practices

Maintaining strict aseptic technique throughout the bacteriostatic water for peptides reconstitution process prevents contamination and ensures research integrity. Always work in a clean, draft-free environment to minimize airborne particle introduction. Use fresh alcohol prep pads for each rubber stopper access, allowing complete evaporation before needle insertion. Never reuse syringes or needles, as this practice introduces contamination risk that even bacteriostatic water cannot overcome. Store reconstituted peptides in the refrigerator at 36-46°F, protecting them from light and temperature fluctuations that accelerate degradation.

The angle and speed of bacteriostatic water injection significantly impact peptide integrity during reconstitution. Insert the needle through the rubber stopper at approximately a 45-degree angle, directing the bac water stream against the vial wall rather than directly onto the lyophilized peptide cake. Inject slowly and steadily, allowing the bacteriostatic water for injection to gently cascade down the wall and gradually dissolve the peptide. Rapid injection or direct impact can cause foaming and peptide denaturation, reducing potency and creating measurement difficulties. Patience during this critical step preserves your valuable peptide compounds.

Documentation practices enhance safety and accuracy when using bacteriostatic water peptides solutions. Label each reconstituted vial with the reconstitution date, peptide concentration, and expiration date (28 days from opening for bac water). Maintain a research log recording peptide batch numbers, bacteriostatic water lot numbers, reconstitution volumes, and storage conditions. This documentation enables troubleshooting if unexpected results occur and ensures compliance with laboratory protocols. Track the number of withdrawals from each bacteriostatic water for injection vial to monitor contamination risk and plan timely replacement before the 28-day limit expires.

STORAGE AND HANDLING GUIDELINES

Proper Storage Conditions

Bacteriostatic water for peptides requires specific storage conditions to maintain its sterile, preservative-protected properties throughout its shelf life. Unopened vials of bac water should be stored at controlled room temperature between 59-86°F (15-30°C), protected from direct sunlight and extreme temperature fluctuations. A dark cabinet or drawer away from heat sources provides ideal storage conditions. The pharmaceutical-grade glass vial and rubber stopper protect bacteriostatic water for injection from environmental contamination, but proper storage prevents degradation of the benzyl alcohol preservative that could compromise antimicrobial effectiveness.

Once opened, bacteriostatic water peptides solutions benefit from refrigeration at 36-46°F (2-8°C), though this is not strictly required. Refrigeration extends the effective life of the benzyl alcohol preservative and provides additional protection against bacterial growth. Store opened bac water vials upright to prevent rubber stopper contact with the liquid, which could cause extractables to leach into the solution. Never freeze bacteriostatic water for injection, as freezing can damage the vial, compromise the rubber stopper seal, and potentially alter the preservative concentration through ice crystal formation.

The storage environment for bacteriostatic water for peptides should remain clean, dry, and free from potential contaminants. Avoid storing bac water near chemicals, cleaning agents, or other substances that could generate vapors or particulates. Keep bacteriostatic water vials in their original packaging until use to protect against light exposure and physical damage. Inspect vials before each use for cracks, cloudiness, or particulate matter. Any bacteriostatic water for injection showing signs of contamination or damage should be discarded immediately, regardless of the expiration date or time since opening.

Shelf Life and Expiration

Understanding bacteriostatic water shelf life ensures optimal performance and safety in peptide reconstitution applications. Unopened vials of bac water for peptides typically maintain full potency for 24-36 months from the manufacturing date when stored under proper conditions. The expiration date printed on each vial reflects the manufacturer’s guarantee of sterility, preservative effectiveness, and pH stability. PrymaLab’s bacteriostatic water for injection undergoes accelerated stability testing to verify that benzyl alcohol concentration and antimicrobial activity remain within specifications throughout the labeled shelf life.

The critical shelf life consideration for bacteriostatic water peptides involves the 28-day usage period after first opening. Once the rubber stopper is punctured, the vial’s sterile integrity depends entirely on the benzyl alcohol preservative to prevent bacterial growth. The 0.9% benzyl alcohol concentration in bac water maintains effective bacteriostatic activity for 28 days under proper storage and handling conditions. After this period, preservative effectiveness may decline, and contamination risk increases. Mark the opening date on each bacteriostatic water for injection vial and discard any remaining solution after 28 days, even if the vial appears clear and uncontaminated.

Several factors can shorten the effective shelf life of bacteriostatic water for peptides after opening. Frequent access increases contamination risk, as each needle insertion introduces potential bacterial introduction. Poor storage conditions, particularly exposure to heat or direct sunlight, accelerate benzyl alcohol degradation and reduce preservative effectiveness. Contaminated needles or syringes can overwhelm the bacteriostatic protection that bac water provides. To maximize the 28-day usage period, maintain strict aseptic technique, store opened vials in the refrigerator, and minimize unnecessary access. These practices ensure that bacteriostatic water for injection maintains its protective properties throughout the entire usage period.

Handling and Safety Precautions

Safe handling of bacteriostatic water for peptides begins with understanding proper aseptic technique and contamination prevention. Always wash hands thoroughly before handling bac water vials, and consider wearing clean gloves for additional protection. Use fresh alcohol prep pads to disinfect the rubber stopper before each access, allowing complete alcohol evaporation before needle insertion. This simple practice removes surface contaminants that could be introduced into the bacteriostatic water for injection during withdrawal. Never touch the needle tip or allow it to contact non-sterile surfaces, as this compromises the sterile barrier that protects your reconstituted peptides.

The benzyl alcohol preservative in bacteriostatic water peptides solutions requires specific safety considerations. While the 0.9% concentration is safe for adult use in research applications, bac water should never be used in neonates, infants, or pregnant women due to benzyl alcohol toxicity concerns. The preservative can cause serious adverse effects including metabolic acidosis and neurological complications in these vulnerable populations. For adult research applications, bacteriostatic water for injection poses minimal risk when used as directed. However, individuals with known benzyl alcohol sensitivity should avoid bac water and use sterile water for single-dose applications instead.

Disposal of used bacteriostatic water for peptides follows standard laboratory waste protocols. Expired or contaminated bac water vials should be discarded in appropriate pharmaceutical waste containers, not poured down drains or disposed of in regular trash. Used syringes and needles must go into puncture-resistant sharps containers to prevent injury and disease transmission. Never recap needles after use, as this practice causes most needle-stick injuries. Maintain a clean workspace by immediately disposing of used materials and wiping surfaces with appropriate disinfectants. These safety practices protect both researchers and the environment from potential hazards associated with bacteriostatic water for injection and reconstituted peptides.

Temperature Control and Monitoring

Maintaining proper temperature control for bacteriostatic water peptides ensures preservative effectiveness and solution stability throughout the usage period. Room temperature storage (59-86°F) suffices for unopened bac water vials, but refrigeration at 36-46°F provides optimal conditions for opened vials. Use a dedicated laboratory refrigerator with accurate temperature monitoring rather than a standard household refrigerator that experiences frequent temperature fluctuations from door openings. Place bacteriostatic water for injection vials in the main refrigerator compartment, avoiding the door shelves where temperature varies most significantly.

Temperature extremes pose serious risks to bacteriostatic water for peptides integrity. Heat exposure above 86°F accelerates benzyl alcohol degradation, reducing the preservative’s antimicrobial effectiveness and potentially shortening the 28-day usage period. Prolonged heat exposure can also alter the pH of bac water, affecting compatibility with sensitive peptide compounds. Conversely, freezing bacteriostatic water for injection causes ice crystal formation that can crack the glass vial, compromise the rubber stopper seal, and create concentration gradients as water freezes before benzyl alcohol. Never store bac water in freezers or allow it to freeze during shipping or storage.

Temperature monitoring systems help ensure bacteriostatic water peptides maintain optimal storage conditions. Digital thermometers with min/max memory functions track temperature excursions that could compromise bac water quality. For critical research applications, consider refrigerators with alarm systems that alert users to temperature deviations. When transporting bacteriostatic water for injection between locations, use insulated containers with ice packs to maintain stable temperatures. Allow transported bac water to equilibrate to room temperature before use to prevent condensation inside the vial and ensure accurate volume measurements during peptide reconstitution.

QUALITY ASSURANCE AND COMPLIANCE

USP Standards and Testing

PrymaLab’s bacteriostatic water for peptides meets or exceeds all United States Pharmacopeia (USP) standards for Bacteriostatic Water for Injection. Each production batch undergoes comprehensive testing to verify compliance with USP monograph specifications including sterility assurance, bacterial endotoxin limits, particulate matter analysis, and pH verification. The bac water manufacturing process follows Current Good Manufacturing Practices (cGMP) in FDA-registered facilities with full environmental monitoring and quality control documentation. This rigorous approach ensures that every vial of bacteriostatic water for injection delivers pharmaceutical-grade quality for critical peptide reconstitution applications.

Sterility testing of bacteriostatic water peptides solutions employs both direct inoculation and membrane filtration methods to detect any viable microorganisms. Samples from each production batch undergo 14-day incubation in multiple growth media designed to support diverse bacterial and fungal species. Only batches showing no growth in any test sample receive approval for distribution. Bacterial endotoxin testing using the Limulus Amebocyte Lysate (LAL) assay verifies that bac water contains less than 0.5 EU/mL, well below the USP limit. These stringent sterility standards ensure that bacteriostatic water for injection introduces no contamination into valuable peptide research materials.

Chemical analysis of bacteriostatic water for peptides confirms precise benzyl alcohol concentration and optimal pH range. High-performance liquid chromatography (HPLC) measures benzyl alcohol content, verifying the 0.9% (9mg/mL) specification with tight tolerances. pH testing ensures each batch falls within the 4.5-7.0 range, with target pH of 5.7 for optimal peptide compatibility. Particulate matter analysis using light obscuration and microscopic examination confirms that bac water meets USP limits for subvisible particles. Certificate of Analysis (CoA) documents accompany each lot, providing complete testing results and traceability for bacteriostatic water for injection used in research applications.

Manufacturing Standards

The production of bacteriostatic water peptides occurs in state-of-the-art facilities designed specifically for sterile pharmaceutical manufacturing. ISO Class 7 cleanrooms with ISO Class 5 laminar flow hoods provide the controlled environment necessary for aseptic processing. All personnel undergo extensive training in aseptic technique, gowning procedures, and contamination control. Environmental monitoring programs continuously track airborne particles, surface contamination, and personnel hygiene to ensure bac water manufacturing maintains the highest sterility standards. These controlled conditions prevent contamination during the critical steps of bacteriostatic water for injection production.

Raw material qualification ensures that every component of bacteriostatic water for peptides meets pharmaceutical-grade specifications. The sterile water base originates from multi-stage purification systems producing Water for Injection (WFI) quality. Benzyl alcohol undergoes rigorous testing for purity, with specifications requiring minimum 99% purity and strict limits on potential contaminants. Type I borosilicate glass vials receive inspection for defects, dimensional accuracy, and chemical resistance. Rubber stoppers undergo extractables testing to verify they won’t leach compounds into bac water during storage. Only materials meeting these stringent specifications enter bacteriostatic water for injection production.

Process validation demonstrates that bacteriostatic water peptides manufacturing consistently produces products meeting all quality specifications. Three consecutive validation batches undergo intensive testing and monitoring to verify that every process parameter remains within acceptable ranges. Ongoing process verification continues throughout commercial production, with regular testing confirming that bac water maintains its sterile, preservative-protected properties. Equipment qualification programs ensure that filling machines, autoclaves, and other critical equipment operate correctly. This comprehensive validation approach guarantees that every vial of bacteriostatic water for injection delivers the quality researchers depend on for peptide reconstitution.

Batch Tracking and Traceability

Complete traceability systems track bacteriostatic water for peptides from raw material receipt through final distribution. Each production batch receives a unique lot number that links to comprehensive manufacturing records documenting every step of the production process. These records include raw material lot numbers, equipment used, personnel involved, environmental monitoring data, and all quality control test results. When researchers purchase bac water from PrymaLab, the lot number on their vial connects to this complete manufacturing history, enabling rapid investigation if any quality concerns arise.

Certificate of Analysis (CoA) documents provide detailed quality information for each bacteriostatic water peptides batch. These certificates list all testing performed, including sterility results, endotoxin levels, pH measurements, benzyl alcohol concentration, and particulate matter analysis. The CoA also includes manufacturing and expiration dates, storage recommendations, and regulatory compliance statements. Researchers can request CoAs for their specific bac water lot numbers to verify that their bacteriostatic water for injection meets all specifications. This transparency ensures confidence in product quality for critical peptide reconstitution applications.

Stability testing programs monitor bacteriostatic water for peptides throughout its shelf life to verify that quality attributes remain within specifications. Real-time stability studies store bac water samples under recommended conditions and test them at regular intervals throughout the labeled shelf life. Accelerated stability studies expose samples to elevated temperatures to predict long-term stability and identify potential degradation pathways. These studies confirm that benzyl alcohol concentration, pH, sterility, and other critical parameters remain stable, supporting the labeled expiration date for bacteriostatic water for injection. Ongoing stability monitoring ensures that quality claims remain valid throughout the product lifecycle.

FREQUENTLY ASKED QUESTIONS

General Questions About Bacteriostatic Water

What is bacteriostatic water used for?

Bacteriostatic water for peptides serves as the preferred reconstitution solution for lyophilized peptide compounds in research applications. The 0.9% benzyl alcohol preservative in bac water inhibits bacterial growth, enabling safe multi-dose use over 28 days. Researchers use bacteriostatic water for injection to dissolve peptide powders, creating solutions suitable for precise dosing in various research protocols. The multi-dose capability makes bac water more cost-effective and convenient than single-use sterile water for extended research projects.

How long does bacteriostatic water last after opening?

Opened bacteriostatic water peptides solutions maintain their sterile, preservative-protected properties for 28 days when stored properly. The benzyl alcohol preservative in bac water provides effective antimicrobial protection throughout this period, preventing bacterial growth from repeated needle insertions. After 28 days, preservative effectiveness may decline, and contamination risk increases. Always mark the opening date on your bacteriostatic water for injection vial and discard any remaining solution after 28 days, even if it appears clear and uncontaminated.

Does bacteriostatic water need to be refrigerated?

Unopened bacteriostatic water for peptides can be stored at room temperature (59-86°F) until the expiration date. Once opened, bac water benefits from refrigeration at 36-46°F, though this is not strictly required. Refrigeration helps preserve benzyl alcohol effectiveness and provides additional protection against bacterial growth during the 28-day usage period. Never freeze bacteriostatic water for injection, as freezing can damage the vial and compromise the rubber stopper seal. Store opened bac water upright in the refrigerator, protected from light and temperature fluctuations.

Is bacteriostatic water the same as sterile water?

No, bacteriostatic water peptides solutions differ significantly from sterile water. Sterile water contains only purified water with no preservatives, requiring single-use and immediate discard after opening. Bacteriostatic water for injection contains 0.9% benzyl alcohol preservative that inhibits bacterial growth, enabling safe multi-dose use for 28 days. This fundamental difference makes bac water more practical and cost-effective for research protocols requiring multiple peptide reconstitutions. However, bacteriostatic water should not be used in neonates or infants due to benzyl alcohol toxicity concerns in these populations.

Where can I buy bacteriostatic water?

PrymaLab offers pharmaceutical-grade bacteriostatic water for peptides in convenient 3ML vials. Our bac water meets all USP standards for Bacteriostatic Water for Injection, with complete testing documentation and lot-specific Certificates of Analysis available. Purchase bacteriostatic water for injection directly from PrymaLab to ensure you receive authentic, properly stored product with full traceability. We ship bac water with appropriate temperature control to maintain quality during transit, and our customer service team can answer questions about proper storage and handling.

Reconstitution and Usage Questions

How much bacteriostatic water should I add to peptides?

The volume of bacteriostatic water for peptides depends on your peptide amount and desired concentration. Common ratios include 2mL bac water for 5mg peptide (2.5mg/mL concentration) or 2mL bacteriostatic water for injection for 10mg peptide (5mg/mL concentration). Use a peptide calculator to determine exact volumes based on your specific peptide amount and target dose. Higher concentrations minimize injection volumes but require more precise measurement, while lower concentrations offer easier dosing but larger injection volumes. The 3ML volume of PrymaLab’s bac water accommodates various reconstitution needs.

Can I use bacteriostatic water for all peptides?

Most research peptides reconstitute effectively with bacteriostatic water for peptides. Growth hormone peptides, healing peptides like BPC-157 and TB-500, and metabolic peptides including GLP-1 analogs all work well with bac water. The 0.9% benzyl alcohol preservative and pH range of 4.5-7.0 provide compatibility with most peptide structures. However, some specialized peptides may require specific reconstitution solutions or pH adjustments. Always consult your peptide supplier’s reconstitution recommendations and consider peptide stability requirements when selecting bacteriostatic water for injection versus alternative solutions.

How do I know if my bacteriostatic water is contaminated?

Contaminated bacteriostatic water peptides solutions typically show visible signs including cloudiness, color change, or particulate matter floating in the liquid. Fresh bac water should appear crystal clear with no visible particles or discoloration. If your bacteriostatic water for injection develops any of these signs, discard it immediately regardless of the time since opening. Also discard bac water that has been open longer than 28 days, even if it appears clear, as preservative effectiveness declines after this period. Maintaining strict aseptic technique during each access minimizes contamination risk.

Can I mix different peptides in the same bacteriostatic water?

No, never mix different peptides in the same bacteriostatic water for peptides solution. Each peptide should be reconstituted in its own vial to prevent chemical interactions, maintain accurate dosing, and preserve peptide stability. Different peptides have varying stability profiles, optimal pH ranges, and storage requirements that may be incompatible. Use separate syringes for each peptide to avoid cross-contamination. If your research protocol requires multiple peptides, reconstitute each separately with bac water and administer them individually. This practice ensures optimal potency and prevents unexpected interactions between peptide compounds.

What syringe size should I use with bacteriostatic water?

Insulin syringes (1mL capacity with 100 unit markings) work best for bacteriostatic water for peptides reconstitution and dosing. These syringes provide precise measurement capability essential for accurate peptide dosing, with each unit representing 0.01mL. The fine needle (typically 29-31 gauge) minimizes rubber stopper damage during repeated bac water withdrawals. For larger volume transfers, 3mL syringes with 0.1mL graduations offer adequate precision. Always use fresh, sterile syringes for each bacteriostatic water for injection access to maintain sterility and prevent contamination of your valuable peptide solutions.

Storage and Safety Questions

What happens if bacteriostatic water freezes?

Freezing bacteriostatic water for peptides can cause serious quality problems. Ice crystal formation may crack the glass vial, compromise the rubber stopper seal, and create concentration gradients as water freezes before benzyl alcohol. Frozen and thawed bac water may show altered pH, reduced preservative effectiveness, and potential contamination from seal compromise. Never intentionally freeze bacteriostatic water for injection, and discard any bac water that has accidentally frozen. Protect bac water from freezing during winter shipping by using insulated packaging and monitoring delivery conditions.

Can I use expired bacteriostatic water?

No, never use expired bacteriostatic water for peptides. The expiration date reflects the manufacturer’s guarantee of sterility, preservative effectiveness, and pH stability. After expiration, benzyl alcohol concentration may decline, reducing antimicrobial protection and increasing contamination risk. The pH may drift outside the optimal range, potentially affecting peptide stability after reconstitution. Expired bac water may also develop particulate matter or microbial growth. Always check expiration dates before using bacteriostatic water for injection, and maintain proper inventory rotation to use oldest stock first.

Is bacteriostatic water safe for injection?

Yes, bacteriostatic water for peptides is safe for injection when used properly in adult research applications. The 0.9% benzyl alcohol concentration provides effective antimicrobial protection without causing tissue irritation at typical injection sites. However, bac water should never be used in neonates, infants, or pregnant women due to benzyl alcohol toxicity concerns. For adult use, bacteriostatic water for injection poses minimal risk when administered according to proper protocols. Always use sterile technique, verify product quality, and follow appropriate dosing guidelines for your specific research application.

How should I dispose of used bacteriostatic water?

Dispose of expired or contaminated bacteriostatic water for peptides according to pharmaceutical waste protocols. Pour unused bac water down the drain with plenty of water, as the dilute benzyl alcohol concentration poses no environmental hazard. Discard empty vials in appropriate pharmaceutical waste containers, not regular trash. Used syringes and needles must go into puncture-resistant sharps containers to prevent injury. Never recap needles after use. Follow your institution’s waste disposal guidelines for bacteriostatic water for injection and associated materials to ensure safe, compliant disposal practices.

Can I travel with bacteriostatic water?

Yes, you can travel with bacteriostatic water for peptides, but take precautions to maintain proper storage conditions. Pack bac water in insulated containers with ice packs to prevent temperature extremes during transit. Keep bacteriostatic water for injection in carry-on luggage when flying to avoid cargo hold temperature fluctuations and potential freezing. Ensure vials are well-protected against breakage with bubble wrap or foam padding. Check destination regulations regarding bacteriostatic water transport, as some locations may have restrictions. Upon arrival, immediately transfer bac water to appropriate storage conditions to maintain quality throughout your research period.

TECHNICAL SPECIFICATIONS

Product Specifications

Product Name: Bacteriostatic Water for Injection, USP
Brand: PrymaLab
Volume: 3ML per vial
Active Ingredient: Benzyl Alcohol 0.9% (9mg/mL)
Base Solution: Sterile Water for Injection, USP
pH Range: 4.5 – 7.0 (target 5.7)
Osmolality: Hypotonic
Appearance: Clear, colorless liquid
Particulate Matter: Meets USP <788> requirements
Bacterial Endotoxins: <0.5 EU/mL
Sterility: Meets USP <71> sterility requirements

Packaging Specifications

Primary Container: Type I Borosilicate Glass Vial
Closure System: Pharmaceutical-grade Rubber Stopper
Seal: Aluminum Crimp with Flip-off Cap
Labeling: Lot number, expiration date, storage conditions
Package Insert: Complete usage and safety information
Secondary Packaging: Protective carton with product information

Storage and Handling Specifications

Unopened Storage: 59-86°F (15-30°C)
Opened Storage: 36-46°F (2-8°C) recommended
Shelf Life (Unopened): 24-36 months from manufacture
Shelf Life (Opened): 28 days maximum
Light Protection: Store in original carton until use
Freezing: Do not freeze
Transportation: Maintain 36-77°F (2-25°C) during shipping

Regulatory and Quality Specifications

Regulatory Status: USP Monograph Compliant
Manufacturing: cGMP Certified Facility
Quality System: ISO 13485 Certified
Testing: Batch-specific Certificate of Analysis
Traceability: Complete lot tracking system
Stability: Validated through expiration date
Documentation: Full manufacturing records available

RELATED PRODUCTS AND ACCESSORIES

Complementary PrymaLab Products

Sterile Water for Injection 3ML
Single-use sterile water for applications requiring preservative-free reconstitution. Ideal for neonatal use or when benzyl alcohol sensitivity is a concern. Meets USP standards for Water for Injection.

Insulin Syringes 1mL (100 pack)
Precision syringes with 100 unit markings for accurate bacteriostatic water and peptide dosing. 29-gauge needles minimize rubber stopper damage. Sterile, single-use design ensures contamination prevention.

Alcohol Prep Pads (200 pack)
Sterile 70% isopropyl alcohol pads for disinfecting vial stoppers before bacteriostatic water withdrawal. Individual packaging maintains sterility. Essential for maintaining aseptic technique.

Sharps Container 1 Quart
Puncture-resistant disposal container for used syringes and needles. Meets OSHA standards for safe sharps disposal. Prevents needle-stick injuries and ensures proper waste management.

Digital Refrigerator Thermometer
Accurate temperature monitoring for bacteriostatic water storage. Min/max memory tracks temperature excursions. Alarm function alerts to temperature deviations that could compromise product quality.

Popular Peptide Products

BPC-157 5mg
Healing peptide for tissue repair research. Reconstitutes with 2-2.5mL bacteriostatic water for optimal concentration. Stable for 28 days when stored properly with bac water preservative protection.

TB-500 5mg
Recovery peptide for inflammation and healing research. Compatible with bacteriostatic water reconstitution. Extended research protocols benefit from bac water’s multi-dose capability.

Ipamorelin 5mg
Growth hormone releasing peptide for metabolic research. Requires precise dosing enabled by bacteriostatic water reconstitution. Multi-week protocols utilize bac water’s 28-day stability.

Semaglutide 5mg
GLP-1 analog for metabolic research applications. Sensitive peptide structure benefits from bacteriostatic water’s controlled pH. Extended dosing schedules require bac water’s preservative protection.

Melanotan 2 10mg
Peptide for pigmentation research. Higher concentration vials work well with 2-3mL bacteriostatic water. Cost-effective research enabled by bac water’s multi-dose capability.

COMPLIANCE AND REGULATORY INFORMATION

Intended Use Statement

PrymaLab Bacteriostatic Water for Injection is intended for use as a sterile, preservative-containing diluent for reconstitution of lyophilized peptides and other compounds in research applications. This product is manufactured in accordance with USP standards for Bacteriostatic Water for Injection and complies with all applicable FDA regulations for pharmaceutical-grade reconstitution solutions. Bacteriostatic water for peptides is designed for multi-dose use over a 28-day period when proper aseptic technique and storage conditions are maintained.

Contraindications and Warnings

CONTRAINDICATIONS:

• Do not use in neonates or infants
• Do not use in pregnant or nursing women
• Do not use in individuals with known benzyl alcohol sensitivity
• Do not use for epidural or intrathecal injection
• Do not use after 28 days from first opening
• Do not use if solution is cloudy or contains particulates

WARNINGS:

• Benzyl alcohol has been associated with serious adverse events and death in neonates
• Use only with proper aseptic technique to prevent contamination
• Discard unused portion after 28 days from first opening
• Do not freeze – freezing may compromise container integrity
• Inspect visually for particulate matter and discoloration before each use
• For research use only – not for human therapeutic use

Quality Certifications

PrymaLab maintains comprehensive quality management systems ensuring bacteriostatic water for peptides meets the highest pharmaceutical standards. Our manufacturing facilities hold current Good Manufacturing Practice (cGMP) certification from the FDA, demonstrating compliance with federal regulations for pharmaceutical production. ISO 13485 certification verifies our quality management system meets international standards for medical device and pharmaceutical manufacturing. These certifications require regular audits and continuous improvement programs that ensure consistent product quality.

All bacteriostatic water for injection batches undergo testing in accordance with USP monograph specifications. Our quality control laboratory maintains ISO/IEC 17025 accreditation, confirming technical competence for all analytical testing procedures. Third-party testing laboratories provide independent verification of critical quality attributes including sterility, endotoxin levels, and preservative concentration. This multi-layered testing approach ensures that every vial of bac water meets or exceeds all quality specifications before release for distribution.

Legal and Regulatory Compliance

PrymaLab Bacteriostatic Water for Peptides is manufactured in FDA-registered facilities operating under current Good Manufacturing Practices (cGMP). All production processes, quality control procedures, and documentation systems comply with 21 CFR Part 211 regulations for pharmaceutical manufacturing. Our bac water meets USP monograph specifications for Bacteriostatic Water for Injection, ensuring consistency with recognized pharmaceutical standards. Complete batch records and Certificates of Analysis provide full traceability and regulatory compliance documentation.

This product is intended for research applications only and is not approved for human therapeutic use. Researchers must comply with all applicable local, state, and federal regulations governing the use of bacteriostatic water for injection in their specific applications. Proper handling, storage, and disposal procedures must follow institutional guidelines and regulatory requirements. PrymaLab provides complete product information, safety data sheets, and technical support to assist researchers in maintaining regulatory compliance throughout their research protocols.

CONCLUSION

PrymaLab’s Bacteriostatic Water for Peptides 3ML represents the gold standard for peptide reconstitution in research applications. The pharmaceutical-grade formulation containing 0.9% benzyl alcohol preservative enables safe, cost-effective multi-dose use over 28 days, distinguishing bac water from single-use sterile water alternatives. Rigorous USP compliance, comprehensive quality testing, and cGMP manufacturing ensure consistent product quality that researchers depend on for critical peptide research protocols.

The 3ML volume provides optimal capacity for multiple peptide reconstitutions while maintaining compact storage requirements. Whether working with growth hormone peptides, healing compounds like BPC-157, or metabolic peptides such as GLP-1 analogs, bacteriostatic water for injection delivers reliable performance across diverse research applications. The controlled pH range, sterile formulation, and proven stability profile make bac water the preferred choice for researchers who demand pharmaceutical-grade quality in their reconstitution solutions.

Proper storage, handling, and aseptic technique maximize the benefits of bacteriostatic water peptides solutions. Following the guidelines in this document ensures optimal product performance, maintains sterility throughout the 28-day usage period, and supports accurate, reproducible research results. PrymaLab stands behind every vial of bacteriostatic water for injection with complete quality documentation, technical support, and commitment to pharmaceutical excellence that researchers trust for their most important peptide research projects.