SECTION 1: INTRODUCTION

Erythropoietin (EPO), also known as recombinant human erythropoietin (rhEPO), is a hematopoietic glycoprotein cytokine widely utilized as a laboratory grade research peptide/protein. Classified as a hematopoietic growth factor, EPO is central to scientific research on hypoxia signaling, cellular stress responses, and neurobiological resilience. EPO (Erythropoietin) 3000 IU – Lab Research Supply offers a high purity peptide format for rigorous peptide research and in-vitro studies.

SECTION 2: RESEARCH APPLICATIONS

• Investigated in studies examining HIF-1α signaling, hypoxic adaptation, and erythroid lineage pathways in cell-based systems.
• Research has explored its role in modulating inflammatory cytokine networks and NF-κB/JNK signaling in immune-relevant models.
• In-vitro studies have demonstrated EPOR-mediated JAK2/STAT5 activation and downstream anti-apoptotic signaling cascades.
• Animal model research suggests context-dependent neuroprotective mechanisms, synaptic integrity support, and reduced oxidative stress markers.
• Laboratory investigations focus on angiogenesis, endothelial cell function, and nitric oxide bioavailability under hypoxic stress.
• Studies also assess mitochondrial dynamics, metabolic reprogramming, and tissue recovery biomarkers following experimental injury.

SECTION 3: TECHNICAL SPECIFICATIONS

Purity: ≥98% by HPLC and SDS-PAGE; endotoxin <1.0 EU/µg (LAL). Form: lyophilized powder, sterile-filtered, carrier-free. Content: 3000 IU per vial; bioactivity referenced to WHO international standard. Molecular weight: ~30.4 kDa (polypeptide core); typical glycosylated forms ~34 kDa. Length: 165 amino acids; N-linked glycosylation at Asn24/Asn38/Asn83 and O-linked at Ser126. Appearance: white to off-white lyophilized cake. Solubility: reconstitute in sterile water or buffer with 0.1% carrier protein (e.g., BSA/HSA). CAS: 11096-26-7 (erythropoietin). Designed as a high purity peptide/protein research chemical for scientific research.

SECTION 4: QUALITY ASSURANCE

Produced under ISO 9001–aligned, GMP-like controls for research-grade materials. Each lot undergoes identity and purity confirmation by HPLC, LC-MS, and SDS-PAGE, with endotoxin screening and sterility checks where applicable. A detailed Certificate of Analysis is available for every batch, documenting potency (IU), purity profile, and specifications. Comprehensive lot traceability and validated cold-chain packaging support reproducibility in peptide research.

SECTION 5: STORAGE & HANDLING

Store lyophilized vials at -20 to -80°C, desiccated and protected from light. Stable for at least 12 months lyophilized under recommended conditions. Reconstitute with sterile water or PBS containing 0.1% carrier protein; mix gently, do not vortex. Aliquot to avoid repeated freeze–thaw cycles. After reconstitution: 2–8°C for up to 1 week, -80°C for up to 3 months. Use aseptic technique and appropriate PPE during handling and in-vitro studies.

SECTION 6: COMPLIANCE & DISCLAIMERS

This product is intended exclusively for laboratory research use by qualified professionals. Not approved for human consumption, veterinary applications, or clinical use. Purchasers must be affiliated with a research institution or possess appropriate qualifications. All research must comply with applicable local, state, and federal regulations. The statements regarding research applications have not been evaluated by the FDA. For research use only; not a drug, diagnostic, or household item. Users are responsible for risk assessment and adherence to institutional safety policies.