Preloaded Autoinjector | Ovagen | 3ml Pen | 6mg

Ovagen is Glu-Asp-Leu (EDL), a three-residue synthetic peptide from the Khavinson bioregulator series with a theoretical mass of 375.4 Da. In this listing it comes already dissolved in a preloaded pen: a 3 mL cartridge holding 6 mg of Ovagen at 0.2% w/v, which is 2 mg/mL. Published work on EDL amounts to a few kidney cell and tissue-culture studies from one St Petersburg group, set against that group’s broader idea that short peptides regulate genes.

Specifications

What is in the pen

The cartridge holds 3 mL of sterile solution. The peptide is the same H-EDL-OH sold as freeze-dried powder in the Ovagen 20 mg vial, here at 0.2% w/v: 6 mg per pen, or roughly 16 µmol. The solvent is bacteriostatic water with 0.9% benzyl alcohol as the preservative. On the safety data sheet the peptide is not classified as hazardous, while benzyl alcohol carries statements for harm if swallowed or inhaled, serious eye irritation and possible skin sensitisation.

Keep the pen in its carton at 2 to 8 °C, out of the light, capped between uses and never frozen. The sheet says to discard it if the solution has frozen or looks discoloured or cloudy. The pen holds less peptide than the vial or the spray, which both carry 20 mg. For the general trade-offs between formats, see peptide pen vs vial.

What the research covers

The tripeptide in the pen is coded T-35 in the papers of Khavinson’s group, and the studies that test it directly used kidney cells. In primary kidney cell cultures aged in vitro, the group compared T-35 with the related tripeptide T-31 (AED) and with a calf kidney polypeptide complex; the abstract identifies the gelatinase MMP-14 as the target of T-35, while cell-renewal markers responded to the other two¹. In organotypic kidney cultures from young and old rats, both tripeptides raised proliferation and lowered apoptosis, by less than the kidney complex did².

Behind these sits the group’s general model, in which peptides of two to seven residues reach the cell nucleus, interact with histones and DNA, and alter gene expression and methylation³. That model is stated for short peptides as a class. None of the abstracts measured EDL in a solution like the one in this pen, and none reports its stability in water, so the storage limits on the safety data sheet are the only guidance for the pen itself.

All of this is culture work, and none of it concerns the liver, although Ovagen is often marketed that way. The Ovagen peptide guide covers the wider literature, and the Livagen guide compares the two peptides sold as liver bioregulators.

How each lot is tested

Every lot is tested in PrymaLab’s own laboratory in Ashland, Kentucky before release: RP-HPLC for purity, LC-MS for identity, Karl Fischer titration for water, LAL for endotoxin and USP <71> for sterility. Published certificates are listed on the results page and can be checked by number and key on the verify page. The certificate covers the peptide lot used to fill the pen, not the finished solution. These are in-house results from PrymaLab Analytical, not an independent test, and the lab is not accredited to ISO 17025. The third-party testing guarantee on this page still applies: if any independent laboratory finds the wrong compound, or less than the stated purity or amount, the order is refunded and the cost of the test is reimbursed.

Frequently asked questions

References

1. Khavinson VKh, Lin'kova NS, Polyakova VO, et al. Peptides regulate expression of signaling molecules in kidney cell cultures during in vitro aging. Bull Exp Biol Med. 2014;157(2):261-4. DOI · PubMed
2. Chalisova NI, Lin'kova NS, Nichik TE, et al. Peptide Regulation of Cells Renewal Processes in Kidney Tissue Cultures from Young and Old Animals. Bull Exp Biol Med. 2015;159(1):124-7. DOI · PubMed
3. Khavinson VK, Popovich IG, Linkova NS, et al. Peptide Regulation of Gene Expression: A Systematic Review. Molecules. 2021;26(22). DOI · PubMed