Certificate of Analysis · Lot release · Issued for Epic Wholesalers LLC -dba-Epic Peptides Lab
Thymosin Beta-4 Frag Ac-SDKP 500 mcg Capsules (60 ct)
Lot PL-2610-214 · Capsules · Label claim 500 mcg per capsule
Tested in-house by PrymaLab
In-house analytical result, not an independent third-party test
Lot / batch number
PL-2610-214
Date made
2026-10-07
Date of analysis
2026-10-08
Date issued
2026-10-15
Retest / expiry
2028-10-07
Units in lot / sampled
199 / 2
Storage
—
Certificate type
Lot COA, 2 pages
Compound
Ac-SDKP
Goralatide; N-acetyl-Ser-Asp-Lys-Pro (thymosin beta-4 fragment 1-4)
CAS number
127103-11-1
Molecular formula
C20H33N5O9
Theoretical mass (avg.)
487.51 Da
Sequence
Ac-SDKP
Purity · RP-HPLC, area %
99.65%
Specification ≥ 99.0% • main peak at 6.28 min
Identity · Observed Mass
487.48 Da
Theoretical 487.51 Da • −0.03 Da (62 ppm)
Overall conclusion
PASS
Meets every specification for the tests performed
Test
Method
Specification
Result
Conclusion
Identity (mass)
LC-MS, ESI positive
Theoretical ± 1.0 Da
487.48 Da
PASS
Purity
RP-HPLC, UV 214 nm, area%
≥ 99.0%
99.65%
PASS
Largest impurity
RP-HPLC, area%
≤ 1.0%
0.23% (RT 7.70)
PASS
Capsule content
Content vs label claim
0.45–0.55 mg/cap
0.49 mg/cap
PASS
Bacterial endotoxin
LAL, kinetic
—
—
N/A
Aerobic count (TAMC)
USP <61>
< 10 CFU/g
< 10 CFU/g
PASS
Yeast & mold (TYMC)
USP <61>
< 10 CFU/g
< 10 CFU/g
PASS
E. coli
USP <62>
Absent
Absent
PASS
1HPLC chromatogram
Data file: PL-2610-214-HPLC.raw · Column C18, 150 × 4.6 mm · UV 214 nm
Peak plot drawn from the integrated peak table (area %, log scale); it is not the instrument trace, which is retained in PL-2610-214-HPLC.raw. † Nominal reference retention time for the assigned impurity; the main-peak RT is observed in this lot.
Epic Wholesalers LLC -dba-Epic Peptides Lab
Certificate of Analysis · continued
Thymosin Beta-4 Frag Ac-SDKP 500 mcg Capsules (60 ct) · Lot PL-2610-214 · Certificate PL-26-00326
Lot PL-2610-214
Method, peak table and approval
2HPLC method · PL-2610-214-HPLC.raw
Instrument
Waters Alliance e2695
Data System
Empower 3
Detector
UV absorbance
Detection Wavelength
214 nm
Column
C18, 150 × 4.6 mm, 3.5 µm
Mobile Phase A
0.1% TFA in water
Mobile Phase B
0.1% TFA in MeCN
Gradient
5–95% B over 25 min (linear)
Flow Rate
1.0 mL/min
Column Temperature
40 °C
Injection Volume
10 µL
3Gradient program
Time (min)
% A
% B
0.0
95
5
25.0
5
95
4Peak table
Peak
RT (min)
Area
Area %
1
5.30 †
—
0.08
2
6.28
—
99.65
3
7.10 †
—
0.04
4
7.70 †
—
0.23
Total
—
100.00
Peaks: 1 unidentified impurity; 2 main peak (Ac-SDKP); 3 unidentified impurity; 4 unidentified impurity · Area % from the integrated peak table (absolute areas are not part of the lot-release export) · † nominal reference RT for the assigned impurity · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.
5Identity by mass spectrometry
Instrument
Waters SQD
Ionization / Mode
ESI, positive
Reference m/z (charge)
488.52 (M+H)+
Deconvoluted Mass
487.48 Da
Theoretical Mass
487.51 Da
Difference
−0.03 Da (62 ppm)
Data File
PL-2610-214-MS.d
Acceptance
Within ± 1.0 Da
6Additional tests
Test
Performed by
Date
Report / file
Content vs label claim
PrymaLab Analytical
2026-10-08
—
Microbial limits (USP <61>/<62>)
PrymaLab Analytical
2026-10-14
—
A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.
7Review and approval
D. Reynolds Analyst · performed the testing Date: 2026-10-15
S. Calloway Reviewer · checked against raw data Date: 2026-10-15
M. Phelps Lab Director · approved for release Date: 2026-10-15
