3705 Music Branch Rd, Ashland, KY 41102 · +1 (606) 465-9063 · prymalab.net
Certificate no.
PL-26-00247
Verification key
EX7MSTY4
Scan or enter both at prymalab.net/testing/verify
Certificate of Analysis · Lot release
NAD+ 1000 mg
Lot PL-2610-133 · Lyophilized powder · Label claim 1000 mg per vial
Tested in-house by PrymaLab
In-house analytical result, not an independent third-party test
Lot / batch number
PL-2610-133
Date made
2026-10-05
Date of analysis
2026-10-06
Date issued
2026-10-20
Retest / expiry
2028-10-05
Units in lot / sampled
180 / 2
Storage
−20 °C, tightly sealed, dry, dark
Certificate type
Lot COA, 2 pages
Compound
NAD+
β-Nicotinamide adenine dinucleotide, oxidized (free acid); coenzyme, not a peptide
CAS number
53-84-9
Molecular formula
C21H27N7O14P2
Theoretical mass (avg.)
663.43 Da
Structure
Purity · RP-HPLC, area %
99.60%
Specification ≥ 99.0% • main peak at 6.80 min
Identity · Observed Mass
663.44 Da
Theoretical 663.43 Da • +0.01 Da (15 ppm)
Overall conclusion
PASS
Meets every specification for the tests performed
Test
Method
Specification
Result
Conclusion
Identity (mass)
LC-MS, ESI positive
Theoretical ± 1.0 Da
663.44 Da
PASS
Purity
RP-HPLC, UV 254–306 nm, area%
≥ 99.0%
99.60%
PASS
Largest impurity
RP-HPLC, area%
≤ 1.0%
0.27% (RT 7.60)
PASS
Water content
Karl Fischer
≤ 5.0%
1.62%
PASS
Bacterial endotoxin
LAL, kinetic
≤ 5.0 EU/mg
0.294 EU/mg
PASS
Sterility
USP <71>, 14-day
No growth
No growth
PASS
1HPLC chromatogram
Data file: PL-2610-133-HPLC.raw · Column C18, 150 × 4.6 mm · UV 254–306 nm
Peak plot drawn from the integrated peak table (area %, log scale); it is not the instrument trace, which is retained in PL-2610-133-HPLC.raw. † Nominal reference retention time for the assigned impurity; the main-peak RT is observed in this lot.
PrymaLab
Certificate of Analysis · continued
NAD+ 1000 mg · Lot PL-2610-133 · Certificate PL-26-00247
Lot PL-2610-133
Method, peak table and approval
2HPLC method · PL-2610-133-HPLC.raw
Instrument
Waters Alliance e2695
Data System
Empower 3
Detector
UV absorbance
Detection Wavelength
254–306 nm
Column
C18, 150 × 4.6 mm, 3.5 µm
Mobile Phase A
0.1% TFA in water
Mobile Phase B
0.1% TFA in MeCN
Gradient
5–95% B over 25 min (linear)
Flow Rate
1.0 mL/min
Column Temperature
40 °C
Injection Volume
10 µL
3Gradient program
Time (min)
% A
% B
0.0
95
5
25.0
5
95
4Peak table
Peak
RT (min)
Area
Area %
1
5.70 †
—
0.07
2
6.80
—
99.60
3
7.60 †
—
0.27
4
8.40 †
—
0.06
Total
—
100.00
Peaks: 1 nadh; 2 main peak (NAD+); 3 adp-ribose; 4 unidentified impurity · Area % from the integrated peak table (absolute areas are not part of the lot-release export) · † nominal reference RT for the assigned impurity · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.
5Identity by mass spectrometry
Instrument
Waters SQD
Ionization / Mode
ESI, positive
Reference m/z (ion)
664.44 [M+H]+
Deconvoluted Mass
663.44 Da
Theoretical Mass
663.43 Da
Difference
+0.01 Da (15 ppm)
Data File
PL-2610-133-MS.d
Acceptance
Within ± 1.0 Da
6Additional tests
Test
Performed by
Date
Report / file
Water content (Karl Fischer)
PrymaLab Analytical
2026-10-06
—
Endotoxin (LAL, kinetic)
PrymaLab Analytical
2026-10-06
—
Sterility (USP <71>, day-14 read-out)
PrymaLab Analytical
2026-10-20
—
A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.
7Review and approval
D. Reynolds Analyst · performed the testing Date: 2026-10-20
S. Calloway Reviewer · checked against raw data Date: 2026-10-20
M. Phelps Lab Director · approved for release Date: 2026-10-20
