PrymaLab

PrymaLab Analytical · In-house laboratory

3705 Music Branch Rd, Ashland, KY 41102 · +1 (606) 465-9063 · prymalab.net

Certificate no.

PL-26-00227

Verification key

HWSSK9MU

Scan or enter both at
prymalab.net/testing/verify

Certificate of Analysis · Lot release

KLOW Blend 80 mg/mL Pen

Lot PL-2610-113 · Solution · Label claim 80 mg/mL

Tested in-house by PrymaLab

In-house analytical result,
not an independent third-party test

Lot / batch number

PL-2610-113

Date made

2026-10-05

Date of analysis

2026-10-06

Date issued

2026-10-20

Retest / expiry

2028-10-05

Units in lot / sampled

211 / 2

Storage

—

Certificate type

Lot COA, 2 pages

Compound

KLOW Blend

GHK-Cu + BPC-157 + TB-500 + KPV co-lyophilized blend

CAS number

89030-95-5 · 137525-51-0 · 77591-33-4 · 67727-97-3

Molecular formula

—

Theoretical mass (avg.)

401.91 · 1,419.55 · 4,963.44 · 342.43 Da (GHK-Cu / BPC-157 / TB-500 / KPV)

Composition and sequences

GHK-Cu: GHK-Cu(II) · BPC-157: GEPPPGKPADDAGLV · TB-500: Ac-43-aa Tbeta4 · KPV: KPV

Purity · RP-HPLC, area %

99.40%

Lowest component (BPC-157) • specification ≥ 99.0% for every component

Identity · Components Confirmed

4 / 4

Components confirmed by LC-MS within ± 1.0 Da: 401.89 / 1,419.50 / 4,963.47 / 342.44 Da

Overall conclusion

PASS

Meets every specification for the tests performed

Test	Method	Specification	Result	Conclusion
GHK-Cu: purity · largest impurity · mass	RP-HPLC 214 nm (area%); LC-MS, ESI+	≥ 99.0% · ≤ 1.0% · ± 1.0 Da	99.73% · 0.15% · 401.89 Da	PASS
BPC-157: purity · largest impurity · mass	RP-HPLC 214 nm (area%); LC-MS, ESI+	≥ 99.0% · ≤ 1.0% · ± 1.0 Da	99.40% · 0.44% · 1,419.50 Da	PASS
TB-500: purity · largest impurity · mass	RP-HPLC 214 nm (area%); LC-MS, ESI+	≥ 99.0% · ≤ 1.0% · ± 1.0 Da	99.48% · 0.34% · 4,963.47 Da	PASS
KPV: purity · largest impurity · mass	RP-HPLC 214 nm (area%); LC-MS, ESI+	≥ 99.0% · ≤ 1.0% · ± 1.0 Da	99.82% · 0.10% · 342.44 Da	PASS
Assay (total blend)	Assay vs label claim	76–84 mg/mL	81.93 mg/mL	PASS
Bacterial endotoxin	LAL, kinetic	≤ 5.0 EU/mg	0.285 EU/mg	PASS
Sterility	USP <71>, 14-day	No growth	No growth	PASS

1HPLC chromatogram

Data file: PL-2610-113-HPLC.raw · Column C18, 150 × 4.6 mm · UV 214 nm

Peak plot drawn from the integrated peak table (area %, log scale); it is not the instrument trace, which is retained in PL-2610-113-HPLC.raw. † Nominal reference retention time for the assigned impurity; the main-peak RT is observed in this lot. Area % is per component (each component main peak + its impurities = 100%).

PrymaLab

Certificate of Analysis · continued

KLOW Blend 80 mg/mL Pen · Lot PL-2610-113 · Certificate PL-26-00227

Lot PL-2610-113

Method, peak table and approval

2HPLC method · PL-2610-113-HPLC.raw

Instrument

Waters Alliance e2695

Detection Wavelength

214 nm

Column

C18, 150 × 4.6 mm, 3.5 µm

Mobile Phase A

0.1% TFA in water

Mobile Phase B

0.1% TFA in MeCN

Gradient

5–95% B over 25 min (linear)

Flow Rate

1.0 mL/min

Column Temperature

40 °C

Injection Volume

10 µL

3Gradient program

Time (min)	% A	% B
0.0	95	5
25.0	5	95

4Peak table

Peak	RT (min)	Area	Area %
1	4.70 †	—	0.05
2	5.65	—	99.82
3	6.50 †	—	0.03
4	7.10 †	—	0.10
5	9.30 †	—	0.15
6	10.73	—	99.73
7	11.40 †	—	0.03
8	11.50 †	—	0.04
9	12.20 †	—	0.08
10	12.20 †	—	0.13
11	13.05	—	99.40
12	13.20 †	—	0.05
13	13.90 †	—	0.44
14	13.90 †	—	0.34
15	14.81	—	99.48
16	15.60 †	—	0.13

Main peaks: 2 KPV · 6 GHK-Cu · 11 BPC-157 · 15 TB-500; all other peaks are the assigned impurities of the neighbouring component · area % within each component (main + impurities = 100% per component) · † nominal reference RT · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.

5Identity by mass spectrometry

Instrument

Waters SQD

Ionization / Mode

ESI, positive

Deconvoluted Mass

401.89 / 1,419.50 / 4,963.47 / 342.44 Da

Theoretical Mass

401.91 / 1,419.55 / 4,963.44 / 342.43 Da

Order

GHK-Cu / BPC-157 / TB-500 / KPV

Difference

−0.02 / −0.05 / +0.03 / +0.01 Da

Data File

PL-2610-113-MS.d

Acceptance

Within ± 1.0 Da (each component)

6Additional tests

Test	Performed by	Date	Report / file
Assay vs label claim	PrymaLab Analytical	2026-10-06	—
Endotoxin (LAL, kinetic)	PrymaLab Analytical	2026-10-06	—
Sterility (USP <71>, day-14 read-out)	PrymaLab Analytical	2026-10-20	—

A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.

7Review and approval

D. Reynolds
Analyst · performed the testing
Date: 2026-10-20

S. Calloway
Reviewer · checked against raw data
Date: 2026-10-20

M. Phelps
Lab Director · approved for release
Date: 2026-10-20