Certificate PL-26-00212 · HHB Blend 10 mL · lot PL-2610-096
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PrymaLab
PrymaLab
PrymaLab Analytical · In-house laboratory
3705 Music Branch Rd, Ashland, KY 41102 · +1 (606) 465-9063 · prymalab.net
Certificate no.
PL-26-00212
Verification key
2R5MB7JW
Scan or enter both at
prymalab.net/testing/verify
Certificate of Analysis · Lot release

HHB Blend 10 mL

Lot PL-2610-096 · Solution · Label claim 10 mL/vial
Tested in-house by PrymaLab
In-house analytical result,
not an independent third-party test
Lot / batch number
PL-2610-096
Date made
2026-10-05
Date of analysis
2026-10-06
Date issued
2026-10-21
Retest / expiry
2028-10-05
Units in lot / sampled
415 / 2
Storage
—
Certificate type
Lot COA, 2 pages
Compound
HHB Blend
B-vitamin and lipotropic research blend (9 components)
CAS number
n/a (blend)
Molecular formula
Not applicable (blend)
Theoretical mass (avg.)
n/a
Composition and sequences
Niacinamide 51.135 mg: mg/vial · Thiamine HCl 50.552 mg: mg/vial · Dexpanthenol 24.282 mg: mg/vial · Choline 9.601 mg: mg/vial · Inositol 10.019 mg: mg/vial · Niacin 5.108 mg: mg/vial · Biotin 0.1 mg: mg/vial · Folic acid 0.097 mg: mg/vial · Riboflavin 0.103 mg: mg/vial
Purity · RP-HPLC, area %
—
Not applicable: multi-component blend (no single-compound purity); see content
Total content · HPLC vs standards
151.0 mg
Per vial • specification 142.8–157.8 mg/vial • identity by co-elution with standards
Overall conclusion
PASS
Meets every specification for the tests performed
TestMethodSpecificationResultConclusion
IdentityCo-elution with standardsMatches standardsMatches standardsPASS
Total contentHPLC, UV 254 nm vs standards142.8–157.8 mg/vial151.0 mg/vialPASS
Bacterial endotoxinLAL, kinetic≤ 5.0 EU/mg0.037 EU/mgPASS
SterilityUSP <71>, 14-dayNo growthNo growthPASS
NiacinamideHPLC 254nm vs standardsReported51.135 mg/vial—
Thiamine HClHPLC 254nm vs standardsReported50.552 mg/vial—
DexpanthenolHPLC 254nm vs standardsReported24.282 mg/vial—
CholineHPLC 254nm vs standardsReported9.601 mg/vial—
InositolHPLC 254nm vs standardsReported10.019 mg/vial—
NiacinHPLC 254nm vs standardsReported5.108 mg/vial—
BiotinHPLC 254nm vs standardsReported0.1 mg/vial—
Folic acidHPLC 254nm vs standardsReported0.097 mg/vial—
RiboflavinHPLC 254nm vs standardsReported0.103 mg/vial—

1HPLC chromatogram

Data file: PL-2610-096-HPLC.raw · Column — · UV 254 nm
Blend quantified against reference standards; component amounts are in the results table.
Components identified and quantified against reference standards; amounts are listed in the results table. No peak plot is drawn for this blend.
PrymaLab
PrymaLab
Certificate of Analysis · continued
HHB Blend 10 mL · Lot PL-2610-096 · Certificate PL-26-00212
Lot PL-2610-096
Method, peak table and approval

2HPLC method · PL-2610-096-HPLC.raw

Instrument
Waters Alliance e2695
Data System
Empower 3
Technique
HPLC 254nm vs standards
Column
—
Detection Wavelength
254 nm
Data File
PL-2610-096-HPLC.raw

3Gradient program

Time (min)% A% B

4Peak table

PeakRT (min)AreaArea %
Not applicable: components are quantified against reference standards (amounts in the table on page 1) · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.

5Identity by mass spectrometry

Not applicable
Identity by co-elution with reference standards; no mass-spectrometry test

6Additional tests

TestPerformed byDateReport / file
Endotoxin (LAL, kinetic)PrymaLab Analytical2026-10-06—
Sterility (USP <71>, day-14 read-out)PrymaLab Analytical2026-10-20—
A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.

7Review and approval

D. Reynolds
Analyst · performed the testing
Date: 2026-10-21
S. Calloway
Reviewer · checked against raw data
Date: 2026-10-21
M. Phelps
Lab Director · approved for release
Date: 2026-10-21