Lowest component (TB-500) • specification ≥ 99.0% for every component
Identity · Components Confirmed
3 / 3
Components confirmed by LC-MS within ± 1.0 Da: 401.89 / 1,419.61 / 4,963.71 Da
Overall conclusion
PASS
Meets every specification for the tests performed
Test
Method
Specification
Result
Conclusion
GHK-Cu: purity · largest impurity · mass
RP-HPLC 214 nm (area%); LC-MS, ESI+
≥ 99.0% · ≤ 1.0% · ± 1.0 Da
99.57% · 0.22% · 401.89 Da
PASS
BPC-157: purity · largest impurity · mass
RP-HPLC 214 nm (area%); LC-MS, ESI+
≥ 99.0% · ≤ 1.0% · ± 1.0 Da
99.64% · 0.19% · 1,419.61 Da
PASS
TB-500: purity · largest impurity · mass
RP-HPLC 214 nm (area%); LC-MS, ESI+
≥ 99.0% · ≤ 1.0% · ± 1.0 Da
99.52% · 0.29% · 4,963.71 Da
PASS
Water content
Karl Fischer
≤ 5.0%
1.70%
PASS
Bacterial endotoxin
LAL, kinetic
≤ 5.0 EU/mg
0.462 EU/mg
PASS
Sterility
USP <71>, 14-day
No growth
No growth
PASS
1HPLC chromatogram
Data file: PL-2610-082-HPLC.raw · Column C18, 150 × 4.6 mm · UV 214 nm
Peak plot drawn from the integrated peak table (area %, log scale); it is not the instrument trace, which is retained in PL-2610-082-HPLC.raw. † Nominal reference retention time for the assigned impurity; the main-peak RT is observed in this lot. Area % is per component (each component main peak + its impurities = 100%).
Epic Wholesalers LLC -dba-Epic Peptides Lab
Certificate of Analysis · continued
GLOW Blend 70 mg · Lot PL-2610-082 · Certificate PL-26-00200
Lot PL-2610-082
Method, peak table and approval
2HPLC method · PL-2610-082-HPLC.raw
Instrument
Waters Alliance e2695
Data System
Empower 3
Detector
UV absorbance
Detection Wavelength
214 nm
Column
C18, 150 × 4.6 mm, 3.5 µm
Mobile Phase A
0.1% TFA in water
Mobile Phase B
0.1% TFA in MeCN
Gradient
5–95% B over 25 min (linear)
Flow Rate
1.0 mL/min
Column Temperature
40 °C
Injection Volume
10 µL
3Gradient program
Time (min)
% A
% B
0.0
95
5
25.0
5
95
4Peak table
Peak
RT (min)
Area
Area %
1
9.30 †
—
0.13
2
10.73
—
99.57
3
11.40 †
—
0.19
4
11.50 †
—
0.22
5
12.20 †
—
0.08
6
12.20 †
—
0.08
7
13.07
—
99.64
8
13.20 †
—
0.11
9
13.90 †
—
0.09
10
13.90 †
—
0.29
11
14.81
—
99.52
12
15.60 †
—
0.08
Total
—
100.00 per component
Main peaks: 2 GHK-Cu · 7 BPC-157 · 11 TB-500; all other peaks are the assigned impurities of the neighbouring component · area % within each component (main + impurities = 100% per component) · † nominal reference RT · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.
5Identity by mass spectrometry
Instrument
Waters SQD
Ionization / Mode
ESI, positive
Deconvoluted Mass
401.89 / 1,419.61 / 4,963.71 Da
Theoretical Mass
401.91 / 1,419.55 / 4,963.44 Da
Order
GHK-Cu / BPC-157 / TB-500
Difference
−0.02 / +0.06 / +0.27 Da
Data File
PL-2610-082-MS.d
Acceptance
Within ± 1.0 Da (each component)
6Additional tests
Test
Performed by
Date
Report / file
Water content (Karl Fischer)
PrymaLab Analytical
2026-10-05
—
Endotoxin (LAL, kinetic)
PrymaLab Analytical
2026-10-05
—
Sterility (USP <71>, day-14 read-out)
PrymaLab Analytical
2026-10-19
—
A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.
7Review and approval
D. Reynolds Analyst · performed the testing Date: 2026-10-19
S. Calloway Reviewer · checked against raw data Date: 2026-10-19
M. Phelps Lab Director · approved for release Date: 2026-10-19
