Certificate PL-26-00198 · GLOW Blend 50 mg · lot PL-2610-081
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PrymaLab Analytical · In-house laboratory
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Certificate no.
PL-26-00198
Verification key
78K3DTYQ
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Certificate of Analysis · Lot release

GLOW Blend 50 mg

Lot PL-2610-081 · Lyophilized co-blend powder · Label claim 50 mg/vial
Tested in-house by PrymaLab
In-house analytical result,
not an independent third-party test
Lot / batch number
PL-2610-081
Date made
2026-10-02
Date of analysis
2026-10-05
Date issued
2026-10-19
Retest / expiry
2028-10-02
Units in lot / sampled
241 / 2
Storage
−20 °C, desiccated, dark
Certificate type
Lot COA, 2 pages
Compound
GLOW Blend
GHK-Cu + BPC-157 + TB-500 co-lyophilized blend
CAS number
89030-95-5 · 137525-51-0 · 77591-33-4
Molecular formula
—
Theoretical mass (avg.)
401.91 · 1,419.55 · 4,963.44 Da (GHK-Cu / BPC-157 / TB-500)
Composition and sequences
GHK-Cu: GHK-Cu(II) · BPC-157: GEPPPGKPADDAGLV · TB-500: Ac-43-aa Tbeta4
Purity · RP-HPLC, area %
99.54%
Lowest component (BPC-157) • specification ≥ 99.0% for every component
Identity · Components Confirmed
3 / 3
Components confirmed by LC-MS within ± 1.0 Da: 401.90 / 1,419.62 / 4,963.36 Da
Overall conclusion
PASS
Meets every specification for the tests performed
TestMethodSpecificationResultConclusion
GHK-Cu: purity · largest impurity · massRP-HPLC 214 nm (area%); LC-MS, ESI+≥ 99.0% · ≤ 1.0% · ± 1.0 Da99.66% · 0.20% · 401.90 DaPASS
BPC-157: purity · largest impurity · massRP-HPLC 214 nm (area%); LC-MS, ESI+≥ 99.0% · ≤ 1.0% · ± 1.0 Da99.54% · 0.23% · 1,419.62 DaPASS
TB-500: purity · largest impurity · massRP-HPLC 214 nm (area%); LC-MS, ESI+≥ 99.0% · ≤ 1.0% · ± 1.0 Da99.54% · 0.27% · 4,963.36 DaPASS
Water contentKarl Fischer≤ 5.0%3.27%PASS
Bacterial endotoxinLAL, kinetic≤ 5.0 EU/mg0.240 EU/mgPASS
SterilityUSP <71>, 14-dayNo growthNo growthPASS

1HPLC chromatogram

Data file: PL-2610-081-HPLC.raw · Column C18, 150 × 4.6 mm · UV 214 nm
0.01%0.1%1%10%100%891011121314151617Retention time (min) · stick height = area % (log scale)99.66% · 10.63 min99.54% · 12.98 min99.54% · 14.77 min0.07% †0.23% †0.07% †0.20% †0.17% †0.15% †0.06% †0.04% †0.27% †
Peak plot drawn from the integrated peak table (area %, log scale); it is not the instrument trace, which is retained in PL-2610-081-HPLC.raw. † Nominal reference retention time for the assigned impurity; the main-peak RT is observed in this lot. Area % is per component (each component main peak + its impurities = 100%).
PrymaLab
PrymaLab
Certificate of Analysis · continued
GLOW Blend 50 mg · Lot PL-2610-081 · Certificate PL-26-00198
Lot PL-2610-081
Method, peak table and approval

2HPLC method · PL-2610-081-HPLC.raw

Instrument
Waters Alliance e2695
Data System
Empower 3
Detector
UV absorbance
Detection Wavelength
214 nm
Column
C18, 150 × 4.6 mm, 3.5 µm
Mobile Phase A
0.1% TFA in water
Mobile Phase B
0.1% TFA in MeCN
Gradient
5–95% B over 25 min (linear)
Flow Rate
1.0 mL/min
Column Temperature
40 °C
Injection Volume
10 µL

3Gradient program

Time (min)% A% B
0.0955
25.0595

4Peak table

PeakRT (min)AreaArea %
19.30 †—0.07
210.63—99.66
311.40 †—0.23
411.50 †—0.07
512.20 †—0.20
612.20 †—0.17
712.98—99.54
813.20 †—0.15
913.90 †—0.06
1013.90 †—0.04
1114.77—99.54
1215.60 †—0.27
Total—100.00 per component
Main peaks: 2 GHK-Cu · 7 BPC-157 · 11 TB-500; all other peaks are the assigned impurities of the neighbouring component · area % within each component (main + impurities = 100% per component) · † nominal reference RT · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.

5Identity by mass spectrometry

Instrument
Waters SQD
Ionization / Mode
ESI, positive
Deconvoluted Mass
401.90 / 1,419.62 / 4,963.36 Da
Theoretical Mass
401.91 / 1,419.55 / 4,963.44 Da
Order
GHK-Cu / BPC-157 / TB-500
Difference
−0.01 / +0.07 / −0.08 Da
Data File
PL-2610-081-MS.d
Acceptance
Within ± 1.0 Da (each component)

6Additional tests

TestPerformed byDateReport / file
Water content (Karl Fischer)PrymaLab Analytical2026-10-05—
Endotoxin (LAL, kinetic)PrymaLab Analytical2026-10-05—
Sterility (USP <71>, day-14 read-out)PrymaLab Analytical2026-10-19—
A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.

7Review and approval

D. Reynolds
Analyst · performed the testing
Date: 2026-10-19
S. Calloway
Reviewer · checked against raw data
Date: 2026-10-19
M. Phelps
Lab Director · approved for release
Date: 2026-10-19