Lowest component (BPC-157) • specification ≥ 99.0% for every component
Identity · Components Confirmed
2 / 2
Components confirmed by LC-MS within ± 1.0 Da: 1,419.47 / 4,963.35 Da
Overall conclusion
PASS
Meets every specification for the tests performed
Test
Method
Specification
Result
Conclusion
BPC-157: purity · largest impurity · mass
RP-HPLC 214 nm (area%); LC-MS, ESI+
≥ 99.0% · ≤ 1.0% · ± 1.0 Da
99.56% · 0.16% · 1,419.47 Da
PASS
TB-500: purity · largest impurity · mass
RP-HPLC 214 nm (area%); LC-MS, ESI+
≥ 99.0% · ≤ 1.0% · ± 1.0 Da
99.58% · 0.20% · 4,963.35 Da
PASS
Water content
Karl Fischer
≤ 5.0%
2.80%
PASS
Bacterial endotoxin
LAL, kinetic
≤ 5.0 EU/mg
0.197 EU/mg
PASS
Sterility
USP <71>, 14-day
No growth
No growth
PASS
1HPLC chromatogram
Data file: PL-2610-024-HPLC.raw · Column C18, 150 × 4.6 mm · UV 214 nm
Peak plot drawn from the integrated peak table (area %, log scale); it is not the instrument trace, which is retained in PL-2610-024-HPLC.raw. † Nominal reference retention time for the assigned impurity; the main-peak RT is observed in this lot. Area % is per component (each component main peak + its impurities = 100%).
Epic Wholesalers LLC -dba-Epic Peptides Lab
Certificate of Analysis · continued
Wolverine Blend 5+5 mg · Lot PL-2610-024 · Certificate PL-26-00146
Lot PL-2610-024
Method, peak table and approval
2HPLC method · PL-2610-024-HPLC.raw
Instrument
Waters Alliance e2695
Data System
Empower 3
Detector
UV absorbance
Detection Wavelength
214 nm
Column
C18, 150 × 4.6 mm, 3.5 µm
Mobile Phase A
0.1% TFA in water
Mobile Phase B
0.1% TFA in MeCN
Gradient
5–95% B over 25 min (linear)
Flow Rate
1.0 mL/min
Column Temperature
40 °C
Injection Volume
10 µL
3Gradient program
Time (min)
% A
% B
0.0
95
5
25.0
5
95
4Peak table
Peak
RT (min)
Area
Area %
1
11.40 †
—
0.15
2
12.20 †
—
0.13
3
13.06
—
99.56
4
13.20 †
—
0.15
5
13.90 †
—
0.16
6
13.90 †
—
0.20
7
14.83
—
99.58
8
15.60 †
—
0.07
Total
—
100.00 per component
Main peaks: 3 BPC-157 · 7 TB-500; all other peaks are the assigned impurities of the neighbouring component · area % within each component (main + impurities = 100% per component) · † nominal reference RT · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.
5Identity by mass spectrometry
Instrument
Waters SQD
Ionization / Mode
ESI, positive
Deconvoluted Mass
1,419.47 / 4,963.35 Da
Theoretical Mass
1,419.55 / 4,963.44 Da
Order
BPC-157 / TB-500
Difference
−0.08 / −0.09 Da
Data File
PL-2610-024-MS.d
Acceptance
Within ± 1.0 Da (each component)
6Additional tests
Test
Performed by
Date
Report / file
Water content (Karl Fischer)
PrymaLab Analytical
2026-10-02
—
Endotoxin (LAL, kinetic)
PrymaLab Analytical
2026-10-02
—
Sterility (USP <71>, day-14 read-out)
PrymaLab Analytical
2026-10-16
—
A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.
7Review and approval
D. Reynolds Analyst · performed the testing Date: 2026-10-16
S. Calloway Reviewer · checked against raw data Date: 2026-10-16
M. Phelps Lab Director · approved for release Date: 2026-10-16
