Certificate PL-26-00146 · Wolverine Blend 5+5 mg · lot PL-2610-024
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PrymaLab
PrymaLab Analytical · In-house laboratory
3705 Music Branch Rd, Ashland, KY 41102 · +1 (606) 465-9063 · prymalab.net
Certificate no.
PL-26-00146
Verification key
8JCP9T9T
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Certificate of Analysis · Lot release

Wolverine Blend 5+5 mg

Lot PL-2610-024 · Lyophilized co-blend powder · Label claim 5 + 5 mg/vial
Tested in-house by PrymaLab
In-house analytical result,
not an independent third-party test
Lot / batch number
PL-2610-024
Date made
2026-10-01
Date of analysis
2026-10-02
Date issued
2026-10-16
Retest / expiry
2028-10-01
Units in lot / sampled
307 / 2
Storage
−20 °C, desiccated, dark
Certificate type
Lot COA, 2 pages
Compound
Wolverine Blend
BPC-157 + TB-500 co-lyophilized 1:1 blend
CAS number
137525-51-0 · 77591-33-4
Molecular formula
—
Theoretical mass (avg.)
1,419.55 · 4,963.44 Da (BPC-157 / TB-500)
Composition and sequences
BPC-157: GEPPPGKPADDAGLV · TB-500: Ac-43-aa Tbeta4
Purity · RP-HPLC, area %
99.56%
Lowest component (BPC-157) • specification ≥ 99.0% for every component
Identity · Components Confirmed
2 / 2
Components confirmed by LC-MS within ± 1.0 Da: 1,419.47 / 4,963.35 Da
Overall conclusion
PASS
Meets every specification for the tests performed
TestMethodSpecificationResultConclusion
BPC-157: purity · largest impurity · massRP-HPLC 214 nm (area%); LC-MS, ESI+≥ 99.0% · ≤ 1.0% · ± 1.0 Da99.56% · 0.16% · 1,419.47 DaPASS
TB-500: purity · largest impurity · massRP-HPLC 214 nm (area%); LC-MS, ESI+≥ 99.0% · ≤ 1.0% · ± 1.0 Da99.58% · 0.20% · 4,963.35 DaPASS
Water contentKarl Fischer≤ 5.0%2.80%PASS
Bacterial endotoxinLAL, kinetic≤ 5.0 EU/mg0.197 EU/mgPASS
SterilityUSP <71>, 14-dayNo growthNo growthPASS

1HPLC chromatogram

Data file: PL-2610-024-HPLC.raw · Column C18, 150 × 4.6 mm · UV 214 nm
0.01%0.1%1%10%100%1011121314151617Retention time (min) · stick height = area % (log scale)99.56% · 13.06 min99.58% · 14.83 min0.15% †0.13% †0.15% †0.16% †0.20% †0.07% †
Peak plot drawn from the integrated peak table (area %, log scale); it is not the instrument trace, which is retained in PL-2610-024-HPLC.raw. † Nominal reference retention time for the assigned impurity; the main-peak RT is observed in this lot. Area % is per component (each component main peak + its impurities = 100%).
PrymaLab
PrymaLab
Certificate of Analysis · continued
Wolverine Blend 5+5 mg · Lot PL-2610-024 · Certificate PL-26-00146
Lot PL-2610-024
Method, peak table and approval

2HPLC method · PL-2610-024-HPLC.raw

Instrument
Waters Alliance e2695
Data System
Empower 3
Detector
UV absorbance
Detection Wavelength
214 nm
Column
C18, 150 × 4.6 mm, 3.5 µm
Mobile Phase A
0.1% TFA in water
Mobile Phase B
0.1% TFA in MeCN
Gradient
5–95% B over 25 min (linear)
Flow Rate
1.0 mL/min
Column Temperature
40 °C
Injection Volume
10 µL

3Gradient program

Time (min)% A% B
0.0955
25.0595

4Peak table

PeakRT (min)AreaArea %
111.40 †—0.15
212.20 †—0.13
313.06—99.56
413.20 †—0.15
513.90 †—0.16
613.90 †—0.20
714.83—99.58
815.60 †—0.07
Total—100.00 per component
Main peaks: 3 BPC-157 · 7 TB-500; all other peaks are the assigned impurities of the neighbouring component · area % within each component (main + impurities = 100% per component) · † nominal reference RT · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.

5Identity by mass spectrometry

Instrument
Waters SQD
Ionization / Mode
ESI, positive
Deconvoluted Mass
1,419.47 / 4,963.35 Da
Theoretical Mass
1,419.55 / 4,963.44 Da
Order
BPC-157 / TB-500
Difference
−0.08 / −0.09 Da
Data File
PL-2610-024-MS.d
Acceptance
Within ± 1.0 Da (each component)

6Additional tests

TestPerformed byDateReport / file
Water content (Karl Fischer)PrymaLab Analytical2026-10-02—
Endotoxin (LAL, kinetic)PrymaLab Analytical2026-10-02—
Sterility (USP <71>, day-14 read-out)PrymaLab Analytical2026-10-16—
A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.

7Review and approval

D. Reynolds
Analyst · performed the testing
Date: 2026-10-16
S. Calloway
Reviewer · checked against raw data
Date: 2026-10-16
M. Phelps
Lab Director · approved for release
Date: 2026-10-16