PrymaLab

PrymaLab Analytical · In-house laboratory

3705 Music Branch Rd, Ashland, KY 41102 · +1 (606) 465-9063 · prymalab.net

Certificate no.

PL-26-00057

Verification key

64Z42E7C

Scan or enter both at
prymalab.net/testing/verify

Certificate of Analysis · Lot release

KLOW Blend 80 mg

Lot PL-2609-057 · Lyophilized co-blend powder · Label claim 80 mg/vial

Tested in-house by PrymaLab

In-house analytical result,
not an independent third-party test

Lot / batch number

PL-2609-057

Date made

2026-09-15

Date of analysis

2026-09-16

Date issued

2026-10-03

Retest / expiry

2028-09-15

Units in lot / sampled

250 / 2

Storage

−20 °C, desiccated, dark

Certificate type

Lot COA, 2 pages

Compound

KLOW Blend

GHK-Cu + BPC-157 + TB-500 + KPV co-lyophilized blend

CAS number

89030-95-5 · 137525-51-0 · 77591-33-4 · 67727-97-3

Molecular formula

C₁₄H₂₂CuN₆O₄ · C₆₂H₉₈N₁₆O₂₂ · C₂₁₂H₃₅₀N₅₆O₇₈S · C₁₆H₃₀N₄O₄

Theoretical mass (avg.)

401.9 · 1419.6 · 4963.5 · 342.4 Da (GHK-Cu / BPC-157 / TB-500 (Thymosin β4) / KPV)

Composition and sequences

GHK-Cu 50 mg: H-GHK-OH · Cu(II) (1:1 copper complex) · BPC-157 10 mg: H-GEPPPGKPADDAGLV-OH (15 residues; free N- and C-termini) · TB-500 (Thymosin β4) 10 mg: Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES-OH (43 residues; N-acetyl; free C-terminus) · KPV 10 mg: H-KPV-OH (3 residues; free N- and C-termini)

Purity · RP-HPLC, area %

99.66%

Specification 99.0% • blend purity — sum of the four component peaks

Identity · Observed Mass

4 / 4

Components confirmed by LC-MS within ± 1.0 Da: 402.1 / 1419.8 / 4963.7 / 342.6 Da

Overall conclusion

PASS

Meets every specification for the tests performed

Test	Method	Specification	Result	Conclusion
Appearance	Visual inspection	Blue to blue-violet lyophilized cake (GHK-Cu copper complex)	Blue lyophilized cake	PASS
Identity (mass)	LC-MS, ESI positive	Theoretical ± 1.0 Da, each component	4/4 components confirmed	PASS
Purity	RP-HPLC, UV 214 nm, area%	99.0% (blend)	99.66%	PASS
Largest impurity	RP-HPLC, area%	1.0%	0.11% at 5.35 min	PASS
Water content	Karl Fischer	5.0%	2.1%	PASS
Bacterial endotoxin	LAL, kinetic	≤ 5.0 EU/mg	0.06 EU/mg	PASS
Sterility / bioburden	USP <71>, membrane filtration	No growth after 14 days	No growth; conforms	PASS

1HPLC chromatogram

Data file: KLOW_PL-2609-057_20260916_01 · Column C18, 150 × 4.6 mm · UV 214 nm

Baseline uncropped; four component peaks and impurities labeled with retention times. Peak table on page 2.

PrymaLab

Certificate of Analysis · continued

KLOW Blend 80 mg · Lot PL-2609-057 · Certificate PL-26-00057

Lot PL-2609-057

Method, peak table and approval

2HPLC method · KLOW_PL-2609-057_20260916_01

Instrument

Waters Alliance e2695, SN E2695B742

Data System

Empower 3, Build 3471

Detector

Waters 2998 PDA

Detection Wavelength

214 nm

Column

C18, 150 × 4.6 mm, 3.5 µm

Column Part / Serial

186003326 / USHX18211

Mobile Phase A

0.1% TFA in water

Mobile Phase B

0.1% TFA in MeCN

Flow Rate

1.0 mL/min

Column Temperature

30 °C

Injection Volume

20 µL

Run Time

10 min

Sample Diluent

Water, 0.1% TFA

Sample Concentration

0.5 mg/mL (blend)

Blank Injected

Yes

Reference Standard

In-house KLOW blend reference, lot PL-RS-KLO-01, component RTs 2.18 / 3.29 / 4.66 / 6.06 min

3Gradient program

Time (min)	% A	% B
0.0	95	5
3.0	95	5
7.0	55	45
8.0	20	80
9.0	20	80
10.0	95	5

4Peak table

Peak	RT (min)	Area	Area %
1 (GHK-Cu)	2.20	2,682,340	62.38
2	2.85	3,870	0.09
3 (KPV)	3.31	525,890	12.23
4 (BPC-157)	4.68	531,910	12.37
5	5.35	4,730	0.11
6 (TB-500)	6.08	545,240	12.68
7	6.90	3,440	0.08
8	7.62	2,580	0.06
Total		4,300,000	100.00

Threshold 0.05% area · Blank subtracted: Y · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.

5Identity by mass spectrometry

Instrument

Waters Acquity SQD

Ionization / Mode

ESI, positive

Observed m/z (charge)

402.8 · 1420.3 (1+ [M+H], GHK-Cu · BPC-157) · 343.2 (1+ [M+H], KPV) · 1241.9 (4+ [M+4H], TB-500) · 993.7 (5+ [M+5H], TB-500)

Deconvoluted Mass

402.1 / 1419.8 / 4963.7 / 342.6 Da (by component)

Theoretical Mass

401.9 / 1419.6 / 4963.5 / 342.4 Da (GHK-Cu / BPC-157 / TB-500 / KPV)

Difference

≤ +0.2 Da (per component)

Data File

KLOW_PL-2609-057_MS_20260916_01

Acceptance

Within ± 1.0 Da (each component)

6Additional tests

Test	Performed by	Date	Report / file
Water content	PrymaLab	2026-09-17	RPT-KF-PL-2609-057
Endotoxin	PrymaLab	2026-09-18	RPT-ET-PL-2609-057
Sterility	PrymaLab	2026-09-30	RPT-ST-PL-2609-057

A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.

7Review and approval

D. Reynolds
Analyst · performed the testing
Date: 2026-09-16

S. Calloway
Reviewer · checked against raw data
Date: 2026-10-02

M. Phelps
Lab Director · approved for release
Date: 2026-10-03