PrymaLab

PrymaLab Analytical · In-house laboratory

3705 Music Branch Rd, Ashland, KY 41102 · +1 (606) 465-9063 · prymalab.net

Certificate no.

PL-26-00041

Verification key

YCC7W5XC

Scan or enter both at
prymalab.net/testing/verify

Certificate of Analysis · Lot release

GKP Blend 70 mg

Lot PL-2609-041 · Lyophilized co-blend powder · Label claim 70 mg/vial

Tested in-house by PrymaLab

In-house analytical result,
not an independent third-party test

Lot / batch number

PL-2609-041

Date made

2026-09-17

Date of analysis

2026-09-19

Date issued

2026-10-03

Retest / expiry

2028-09-17

Units in lot / sampled

250 / 2

Storage

−20 °C, desiccated, dark

Certificate type

Lot COA, 2 pages

Compound

GKP Blend

GHK-Cu + KPV + BPC-157 co-lyophilized blend

CAS number

89030-95-5 · 67727-97-3 · 137525-51-0

Molecular formula

C₁₄H₂₂CuN₆O₄ · C₁₆H₃₀N₄O₄ · C₆₂H₉₈N₁₆O₂₂

Theoretical mass (avg.)

401.9 · 342.4 · 1419.6 Da (GHK-Cu / KPV / BPC-157)

Composition and sequences

GHK-Cu 50 mg: H-GHK-OH · Cu(II) (1:1 copper complex) · KPV 10 mg: H-KPV-OH (3 residues; free N- and C-termini) · BPC-157 10 mg: H-GEPPPGKPADDAGLV-OH (15 residues; free N- and C-termini)

Purity · RP-HPLC, area %

99.71%

Specification 99.0% • blend purity — sum of the three component peaks

Identity · Observed Mass

3 / 3

Components confirmed by LC-MS within ± 1.0 Da: 402.1 / 342.6 / 1419.8 Da

Overall conclusion

PASS

Meets every specification for the tests performed

Test	Method	Specification	Result	Conclusion
Appearance	Visual inspection	Blue to blue-violet lyophilized cake (GHK-Cu copper complex)	Blue lyophilized cake	PASS
Identity (mass)	LC-MS, ESI positive	Theoretical ± 1.0 Da, each component	3/3 components confirmed	PASS
Purity	RP-HPLC, UV 214 nm, area%	99.0% (blend)	99.71%	PASS
Largest impurity	RP-HPLC, area%	1.0%	0.10% at 5.40 min	PASS
Water content	Karl Fischer	5.0%	2.0%	PASS
Bacterial endotoxin	LAL, kinetic	≤ 5.0 EU/mg	0.05 EU/mg	PASS
Sterility / bioburden	USP <71>, membrane filtration	No growth after 14 days	No growth; conforms	PASS

1HPLC chromatogram

Data file: GKP_PL-2609-041_20260919_01 · Column C18, 150 × 4.6 mm · UV 214 nm

Baseline uncropped; three component peaks and impurities labeled with retention times. Peak table on page 2.

PrymaLab

Certificate of Analysis · continued

GKP Blend 70 mg · Lot PL-2609-041 · Certificate PL-26-00041

Lot PL-2609-041

Method, peak table and approval

2HPLC method · GKP_PL-2609-041_20260919_01

Instrument

Waters Alliance e2695, SN E2695B742

Data System

Empower 3, Build 3471

Detector

Waters 2998 PDA

Detection Wavelength

214 nm

Column

C18, 150 × 4.6 mm, 3.5 µm

Column Part / Serial

186003326 / USHX18211

Mobile Phase A

0.1% TFA in water

Mobile Phase B

0.1% TFA in MeCN

Flow Rate

1.0 mL/min

Column Temperature

30 °C

Injection Volume

20 µL

Run Time

10 min

Sample Diluent

Water, 0.1% TFA

Sample Concentration

0.5 mg/mL (blend)

Blank Injected

Yes

Reference Standard

In-house GKP blend reference, lot PL-RS-GKP-01, component RTs 2.18 / 3.29 / 4.66 min

3Gradient program

Time (min)	% A	% B
0.0	95	5
3.0	95	5
7.0	55	45
8.0	20	80
9.0	20	80
10.0	95	5

4Peak table

Peak	RT (min)	Area	Area %
1 (GHK-Cu)	2.20	3,046,550	70.85
2 (KPV)	3.31	519,440	12.08
3 (BPC-157)	4.68	721,540	16.78
4	5.40	4,300	0.10
5	6.15	3,440	0.08
6	7.02	3,870	0.09
Total		4,300,000	100.00

Threshold 0.05% area · Blank subtracted: Y · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.

5Identity by mass spectrometry

Instrument

Waters Acquity SQD

Ionization / Mode

ESI, positive

Observed m/z (charge)

402.8 (1+ [M+H], GHK-Cu) · 343.3 (1+ [M+H], KPV) · 1420.3 (1+ [M+H], BPC-157)

Deconvoluted Mass

402.1 / 342.6 / 1419.8 Da (by component)

Theoretical Mass

401.9 / 342.4 / 1419.6 Da (GHK-Cu / KPV / BPC-157)

Difference

≤ +0.2 Da (per component)

Data File

GKP_PL-2609-041_MS_20260919_01

Acceptance

Within ± 1.0 Da (each component)

6Additional tests

Test	Performed by	Date	Report / file
Water content	PrymaLab	2026-09-20	RPT-KF-PL-2609-041
Endotoxin	PrymaLab	2026-09-21	RPT-ET-PL-2609-041
Sterility	PrymaLab	2026-10-02	RPT-ST-PL-2609-041

A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.

7Review and approval

D. Reynolds
Analyst · performed the testing
Date: 2026-09-19

S. Calloway
Reviewer · checked against raw data
Date: 2026-10-02

M. Phelps
Lab Director · approved for release
Date: 2026-10-03