PrymaLab

PrymaLab Analytical · In-house laboratory

3705 Music Branch Rd, Ashland, KY 41102 · +1 (606) 465-9063 · prymalab.net

Certificate no.

PL-26-00039

Verification key

6LRFJ8AY

Scan or enter both at
prymalab.net/testing/verify

Certificate of Analysis · Lot release

GHRP-6 5 mg

Lot PL-2609-039 · Lyophilized powder · Label claim 5 mg/vial

Tested in-house by PrymaLab

In-house analytical result,
not an independent third-party test

Lot / batch number

PL-2609-039

Date made

2026-09-18

Date of analysis

2026-09-19

Date issued

2026-10-03

Retest / expiry

2028-09-18

Units in lot / sampled

250 / 2

Storage

−20 °C, desiccated, dark

Certificate type

Lot COA, 2 pages

Compound

GHRP-6

Growth hormone-releasing peptide-6

CAS number

87616-84-0

Molecular formula

C₄₆H₅₆N₁₂O₆

Theoretical mass (avg.)

873.0 Da

Sequence

H-H-[D-Trp]-A-W-[D-Phe]-K-NH₂ (6 residues; C-terminal amide)

Purity · RP-HPLC, area %

99.14%

Specification 99.0% • main peak at 5.22 min

Identity · Observed Mass

872.8 Da

Theoretical 873.0 Da • −0.2 Da (229 ppm)

Overall conclusion

PASS

Meets every specification for the tests performed

Test	Method	Specification	Result	Conclusion
Appearance	Visual inspection	White to off-white lyophilized powder	White lyophilized powder	PASS
Identity (mass)	LC-MS, ESI positive	Theoretical ± 1.0 Da	872.8 Da	PASS
Purity	RP-HPLC, UV 214 nm, area%	99.0%	99.14%	PASS
Largest impurity	RP-HPLC, area%	1.0%	0.34% at 4.72 min (des-Trp 3 fragment)	PASS
Water content	Karl Fischer	5.0%	1.6%	PASS
Bacterial endotoxin	LAL, kinetic	≤ 5.0 EU/mg	0.04 EU/mg	PASS
Sterility / bioburden	USP <71>, membrane filtration	No growth after 14 days	No growth; conforms	PASS

1HPLC chromatogram

Data file: GH6_PL-2609-039_20260919_01 · Column C18, 150 × 4.6 mm · UV 214 nm

Baseline uncropped; main peak and impurities labeled with retention times. Peak table on page 2.

PrymaLab

Certificate of Analysis · continued

GHRP-6 5 mg · Lot PL-2609-039 · Certificate PL-26-00039

Lot PL-2609-039

Method, peak table and approval

2HPLC method · GH6_PL-2609-039_20260919_01

Instrument

Waters Alliance e2695, SN E2695B742

Data System

Empower 3, Build 3471

Detector

Waters 2998 PDA

Detection Wavelength

214 nm

Column

C18, 150 × 4.6 mm, 3.5 µm

Column Part / Serial

186003326 / USHX18211

Mobile Phase A

0.1% TFA in water

Mobile Phase B

0.1% TFA in MeCN

Flow Rate

1.0 mL/min

Column Temperature

30 °C

Injection Volume

20 µL

Run Time

10 min

Sample Diluent

Water, 0.1% TFA

Sample Concentration

0.2 mg/mL

Blank Injected

Yes

Reference Standard

In-house GHRP-6 reference, lot PL-RS-GH6-01, RT 5.20 min

3Gradient program

Time (min)	% A	% B
0.0	95	5
3.0	95	5
7.0	55	45
8.0	20	80
9.0	20	80
10.0	95	5

4Peak table

Peak	RT (min)	Area	Area %
1 (des-Trp 3 )	4.72	14,620	0.34
2	5.22	4,263,020	99.14
3 (ox-Trp)	5.94	12,470	0.29
4 (epimer)	6.72	9,890	0.23
Total		4,300,000	100.00

Threshold 0.05% area · Blank subtracted: Y · Purity = main peak area ÷ total integrated area × 100, to two decimals; main peak shaded.

5Identity by mass spectrometry

Instrument

Waters Acquity SQD

Ionization / Mode

ESI, positive

Observed m/z (charge)

874.1 (1+ [M+H]) · 437.7 (2+ [M+2H])

Deconvoluted Mass

872.8 Da (free-base hexapeptide)

Theoretical Mass

873.0 Da

Difference

−0.2 Da (229 ppm)

Data File

GH6_PL-2609-039_MS_20260919_01

Acceptance

Within ± 1.0 Da

6Additional tests

Test	Performed by	Date	Report / file
Water content	PrymaLab	2026-09-20	RPT-KF-PL-2609-039
Endotoxin	PrymaLab	2026-09-21	RPT-ET-PL-2609-039
Sterility	PrymaLab	2026-10-02	RPT-ST-PL-2609-039

A test with no row here was not performed on this lot. Outside-laboratory reports, when used, are attached and named below.

7Review and approval

D. Reynolds
Analyst · performed the testing
Date: 2026-09-19

S. Calloway
Reviewer · checked against raw data
Date: 2026-10-02

M. Phelps
Lab Director · approved for release
Date: 2026-10-03